Effect of Phloretin on the Binding of 1-Anilino-8-naphtalene sulfonate (ANS) to 1,2-Dimyristoyl-sn-glycero-3-phosphocoline (DMPC) Vesicles in the Gel and Liquid-Crystalline State
| Autor: | Guillermo G. Montich, Oscar A. Roveri, Andrea Carmen Cutró |
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| Rok vydání: | 2014 |
| Předmět: |
Physiology
Phloretin Stereochemistry Biophysics ANS binding Quantum yield Anilino Naphthalenesulfonates Ciencias Biológicas chemistry.chemical_compound Phase (matter) Liposome Vesicle Surface potential technology industry and agriculture Cell Biology Biofísica Fluorescence Liquid Crystals Sulfonate Membrane chemistry Liposomes Phase state Dimyristoylphosphatidylcholine Gels CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | The Journal of Membrane Biology. 248:137-144 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/s00232-014-9750-0 |
| Popis: | Phloretin is a known modifier of the internal dipole potential of lipid membranes. We studied the interaction of phloretin with model lipid membranes and how it influences the membrane dipole organization using ANS as fluorescent probe. The fluorescence increase observed when ANS binds to DMPC liposomes in gel phase (13 C) was 2.5 times larger in the presence of phloretin. This effect was due to an increase in ANS affinity, which can be related to the known capability of phloretin in decreasing the dipole potential. Conversely, when the experiments were carried out at 33 C (liquid crystalline phase), phloretin completely inhibited the increase in ANS fluorescence. In addition, phloretin only affected the electrical properties of the membrane in the gel phase, whereas it modifies structural ones in the liquidcrystalline state. We postulate that phloretin was bound only to the DMPC interface in the gel phase decreasing the surface negative charge density without modifying the structural properties of the ANS binding sites. In the liquidcrystalline phase instead, it increased the accessibility of water to the ANS binding sites decreasing the intrinsic affinity and the fluorescence quantum yield of ANS. Fil: Cutró, Andrea Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica Biologica. Area Biofisica; Argentina Fil: Montich, Guillermo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina Fil: Roveri, Oscar Angel. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica Biologica. Area Biofisica; Argentina |
| Databáze: | OpenAIRE |
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