Activated receptor tyrosine kinases in granulosa cells of ovulating follicles in mice
| Autor: | Melissa Pansera, Dayananda Siddappa, Yasmin Schuermann, Raj Duggavathi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ovulation
0301 basic medicine endocrine system medicine.medical_specialty Receptor EphB1 medicine.medical_treatment Biology Fibroblast growth factor Gene Expression Regulation Enzymologic Receptor tyrosine kinase Mice 03 medical and health sciences Epidermal growth factor Internal medicine Genetics medicine Animals Humans Horses Receptor Fibroblast Growth Factor Type 2 Equine chorionic gonadotropin Insulin-like growth factor 1 receptor Granulosa Cells 030102 biochemistry & molecular biology Fibroblast growth factor receptor 2 Growth factor Receptors Somatomedin Cell Biology 030104 developmental biology Endocrinology biology.protein Female Luteinizing hormone Signal Transduction Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 85:316-324 |
| ISSN: | 1040-452X |
| Popis: | Successful ovulation requires the actions of gonadotropins along with those mediated by growth factors binding to their receptor tyrosine kinases (RTKs). There are several growth factors such as epidermal growth factor family ligands and interleukins that play a role during ovulation initiated by the preovulatory surge of luteinizing hormone (LH). The aim of this project was to analyze growth factor signaling pathways induced by LH in mouse granulosa cells. Immature female mice were treated with equine chorionic gonadotropin (eCG) followed 48 hr later by human chorionic gonadotropin (hCG) to induce follicular growth and ovulation. We performed protein array analysis where we identified higher phosphorylation of insulin-like growth factor 1 receptor (IGF1R), the fibroblast growth factor receptor 2 (FGFR2) and ephrin receptor B1 (EPHB1) in granulosa cells at 4 hr post-hCG compared to 0 hr hCG (p < 0.05). We report both a significant increase in transcript abundance (p < 0.05) and the phosphorylation level (p < 0.05) of the IGF1R in granulosa cells at hCG4h. The mRNA abundance of the Fgfr2 and Ephb1 receptors remained unaltered upon hCG treatment. Nonetheless, transcript abundance of the fibroblast growth factor 2 (Fgf2) ligand was elevated at hCG4h (p < 0.01). Based on these results we conclude that the preovulatory LH surge activates signaling pathways of IGF1R through increase in the expression of the Igf1r gene in granulosa cells of ovulating follicles in mice. The LH surge also appears to activate FGFR2 IIIc and EPHB1 signaling, although further investigation is required. |
| Databáze: | OpenAIRE |
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