Some biochemical effects of insulin and steroid hormones on the rat prostate in organ culture
| Autor: | R. Johansson, R.S. Santti |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
medicine.medical_specialty Hydrocortisone medicine.medical_treatment Prednisolone Stimulation Biology Organ culture Tritium Steroid Organ Culture Techniques Internal medicine medicine Animals Insulin Testosterone Amino Acids Cyproterone Protein Precursors Uridine Carbon Isotopes Estradiol Estriol Osmolar Concentration Androstenedione Prostate Cell Biology Carbon Dioxide Culture Media Rats Endocrinology Cholesterol Glucose Protein Biosynthesis RNA Hormone medicine.drug |
| Zdroj: | Experimental cell research. 77(1) |
| ISSN: | 0014-4827 |
| Popis: | The effect of various steroids and insulin on expiants of the ventral prostate of adult rats in organ culture was investigated. Testosterone activated the incorporation of labeled precursors into RNA and protein and the formation of 14CO2 from 14C-glucose by expiants. All these effects were mimiced by insulin. The testosterone action was suppressed by cyproterone in vitro. In addition to androgens, glucocorticoids activated the incorporation of 3H-uridine into RNA. Simultaneous addition of testosterone with hydrocortisone or insulin produced an augmented effect on the incorporation of 3H-uridine into RNA which exceeded that resulting from a simple summation of the individual hormone responses. Insulin facilitated likewise the action of testosterone on the incorporation of 14C-amino acids into protein. When all three hormones were added simultaneously to the culture medium the stimulation of the three biochemical parameters was maximal. It was therefore concluded that all three hormones are necessary for an adequate maintenance of the ventral prostate of the rat. |
| Databáze: | OpenAIRE |
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