Functional male accessory glands and fertility in Drosophila require novel ecdysone receptor
| Autor: | Norene A. Buehner, Anshuman Singh, Anuj K. Pandey, Snigdha Gupta, Kristipati Ravi Ram, Himanshu Pawankumar Gupta, Ajay Kumar, Snigdha Misra, Vandana Sharma |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cancer Research Receptors Steroid Receptors Cytoplasmic and Nuclear Apoptosis Biochemistry 0302 clinical medicine Cell Signaling Animal Cells Electrochemistry Drosophila Proteins Membrane Receptor Signaling Receptor Genetics (clinical) Cell Death Reproduction Animal Models Hormone Receptor Signaling Spermatozoa Insects Chemistry Drosophila melanogaster Experimental Organism Systems Fecundity Hormone receptor Cell Processes Physical Sciences Drosophila Female Cellular Types Coreceptors Research Article Signal Transduction Secondary Cells medicine.medical_specialty lcsh:QH426-470 Arthropoda Biology Research and Analysis Methods 03 medical and health sciences Model Organisms Population Metrics Internal medicine Genetics medicine Animals Molecular Biology Transcription factor Ecology Evolution Behavior and Systematics Infertility Male Homeodomain Proteins Population Biology Organisms Seminal Plasma Proteins Biology and Life Sciences Ecdysteroids Correction Cell Biology biology.organism_classification Invertebrates Sperm Hormones Male accessory gland lcsh:Genetics 030104 developmental biology Endocrinology Germ Cells Electrochemical Cells Fertility Nuclear receptor Ecdysone receptor 030217 neurology & neurosurgery Hormone |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 13, Iss 5, p e1006788 (2017) |
| ISSN: | 1553-7404 1553-7390 |
| Popis: | In many insects, the accessory gland, a secretory tissue of the male reproductive system, is essential for male fertility. Male accessory gland is the major source of proteinaceous secretions, collectively called as seminal proteins (or accessory gland proteins), which upon transfer, manipulate the physiology and behavior of mated females. Insect hormones such as ecdysteroids and juvenoids play a key role in accessory gland development and protein synthesis but little is known about underlying molecular players and their mechanism of action. Therefore, in the present study, we examined the roles of hormone-dependent transcription factors (Nuclear Receptors), in accessory gland development, function and male fertility of a genetically tractable insect model, Drosophila melanogaster. First, we carried out an RNAi screen involving 19 hormone receptors, individually and specifically, in a male reproductive tissue (accessory gland) for their requirement in Drosophila male fertility. Subsequently, by using independent RNAi/ dominant negative forms, we show that Ecdysone Receptor (EcR) is essential for male fertility due to its requirement in the normal development of accessory glands in Drosophila: EcR depleted glands fail to make seminal proteins and have dying cells. Further, our data point to a novel ecdysone receptor that does not include Ultraspiracle but is probably comprised of EcR isoforms in Drosophila male accessory glands. Our data suggest that this novel ecdysone receptor might act downstream of homeodomain transcription factor paired (prd) in the male accessory gland. Overall, the study suggests novel ecdysone receptor as an important player in the hormonal regulation of seminal protein production and insect male fertility. Author summary Insects are the major contributors to biodiversity and have economic, agricultural and health importance. This unparalleled abundance of insects, in part, can be attributed to their high reproductive potential. In many insects, proteins derived from the accessory gland, the secretory tissue of male reproductive system, are critical for fertility. The production of these accessory gland proteins is regulated by insect hormones but the underlying mechanisms/molecular players remain poorly understood. Elucidation of the same has potential applications in designing pest control management strategies and to understand the effect of environmental chemicals on reproduction. In view of this, we analyzed the role, if any, of various insect hormone receptors in development and function of the male accessory gland in a genetically tractable insect model, Drosophila melanogaster. Here, we report the involvement of Ecdysone receptor (EcR with novel composition) in Drosophila male fertility. We show that the depletion of this receptor causes cell death in male accessory glands, which fail to produce seminal fluid proteins leading to sterility/sub-fertility of Drosophila males. These findings will find potential applications in designing insect pest control strategies. |
| Databáze: | OpenAIRE |
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