Immunoglobulin Ihhibiting Reagent®: Evaluation of a New Method for Eliminating Spurious Elevations in CA125 Caused by HAMA
| Autor: | G. Rustin, S. Nicholson, M. Fox, Agamemnon A. Epenetos |
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| Rok vydání: | 1996 |
| Předmět: |
Quality Control
0301 basic medicine Cancer Research Time Factors Anticorps monoclonal medicine.drug_class medicine.medical_treatment Clinical Biochemistry Cancer therapy Antibodies Heterophile Enzyme-Linked Immunosorbent Assay Biology Immunoglobulin E Monoclonal antibody Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Neoplasm Staging Ovarian Neoplasms Antibodies Monoclonal Reproducibility of Results Immunotherapy Radioimmunotherapy 030104 developmental biology Oncology CA-125 Antigen Immunoglobulin G 030220 oncology & carcinogenesis Reagent Immunology biology.protein Female Antibody Artifacts Follow-Up Studies |
| Zdroj: | The International Journal of Biological Markers. 11:46-49 |
| ISSN: | 1724-6008 |
| Popis: | Cancer therapy utilising radiolabelled murine monoclonal antibodies frequently leads to the production of Human Anti-Mouse Antibodies (HAMA) in the recipient. HAMA interferes with “sandwich” immunoassays, such as those for tumour markers, rendering results unreliable. Published methods for eliminating HAMA from serum are not suitable for use in a laboratory which is processing a large number of assays using an automated system. We report on the use of Immunoglobulin Inhibiting Reagent (IIR) in CA125 assays from recipients of intraperitoneal radioimmunotherapy who had spuriously elevated results due to HAMA. IIR was found to be comparable to the admixture of mouse serum as a way of eliminating the effect of HAMA. IIR is ideally suited to use with an automated assay process. |
| Databáze: | OpenAIRE |
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