c-Kit+ cells isolated from human fetal retinas represent a new population of retinal progenitor cells
| Autor: | Peng-Yi Zhou, Zheng Qin Yin, Haiwei Xu, Guang-Hua Peng |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Retinal degeneration Stage-Specific Embryonic Antigens Immunocytochemistry Biology Stem cell marker Retina Flow cytometry Mice chemistry.chemical_compound Fetus medicine Animals Humans Photoreceptor Cells Molecular Biology Cells Cultured Neurons medicine.diagnostic_test Stem Cells Retinal Degeneration Cell Differentiation Retinal Cell Biology Cell sorting Flow Cytometry medicine.disease Embryonic stem cell Molecular biology Rats Transplantation Proto-Oncogene Proteins c-kit medicine.anatomical_structure chemistry Immunology Female sense organs Neuroglia Stem Cell Transplantation Developmental Biology |
| Zdroj: | Development. 142:e1205-e1205 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Definitive surface markers for retinal progenitor cells (RPCs) are still lacking. Therefore, we sorted c-Kit+ and stage-specific embryonic antigen-4− (SSEA4−) retinal cells for further biological characterization. RPCs were isolated from human fetal retinas (gestational age of 12–14 weeks). c-Kit+/SSEA4− RPCs were sorted by fluorescence-activated cell sorting, and their proliferation and differentiation capabilities were evaluated by using immunocytochemistry and flow cytometry. The effectiveness and safety were assessed following injection of c-Kit+/SSEA4− cells into the subretina of Royal College of Surgeons (RCS) rats. c-Kit+ cells were found in the inner part of the fetal retina. Sorted c-Kit+/SSEA4− cells expressed retinal stem cell markers. Our results clearly demonstrate the proliferative potential of these cells. Moreover, c-Kit+/SSEA4− cells differentiated into retinal cells that expressed markers of photoreceptor cells, ganglion cells and glial cells. These cells survived for at least 3 months after transplantation into the host subretinal space. Teratomas were not observed in the c-Kit+/SSEA4−-cell group. Thus, c-Kit can be used as a surface marker for RPCs, and c-Kit+/SSEA4− RPCs exhibit the ability to self-renew and differentiate into retinal cells. |
| Databáze: | OpenAIRE |
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