Gene expression in hippocampus as a function of differential trait anxiety levels in genetically heterogeneous NIH-HS rats

Autor: Rocío Donaire, Carmen Torres, José Antonio Martínez-Conejero, Ignacio Morón, Alberto Fernández-Teruel, Marta Palència, Adolf Tobeña, Regina López-Aumatell, Toni Cañete, Carme Mont-Cardona, Sira Díaz-Morán, Francisco J. Esteban, Esther Martínez-Membrives, Gloria Blázquez, Marta Sabariego
Rok vydání: 2013
Předmět:
Lymphotoxin-beta
Male
Receptors
Vasopressin
Microarray
Organic Anion Transporters
Sodium-Dependent
Nerve Tissue Proteins
Quantitative trait locus
Hippocampal formation
Anxiety
Amygdala
Hippocampus
Plasma Membrane Neurotransmitter Transport Proteins
03 medical and health sciences
Behavioral Neuroscience
Genetic Heterogeneity
0302 clinical medicine
Histocompatibility Antigens
medicine
Avoidance Learning
Animals
Nerve Growth Factors
Maze Learning
Gene
030304 developmental biology
0303 health sciences
Symporters
Genetic heterogeneity
Microarray analysis techniques
Histocompatibility Antigens Class II
Rats
Acid Sensing Ion Channels
Antigens
Differentiation
B-Lymphocyte
Disease Models
Animal
medicine.anatomical_structure
Gene Expression Regulation
Exploratory Behavior
medicine.symptom
Psychology
Neuroscience
030217 neurology & neurosurgery
Zdroj: Behavioural Brain Research; Vol 257
ISSN: 1872-7549
Popis: To identify genes involved in the development/expression of anxiety/fear, we analyzed the gene expression profile in the hippocampus of genetically heterogeneous NIH-HS rats. The NIH-HS rat stock is a unique genetic resource for the fine mapping of quantitative trait loci (QTLs) to very small genomic regions, due to the high amount of genetic recombinants accumulated along more than 50 breeding generations, and for the same reason it can be expected that those genetically heterogeneous rats should be especially useful for studying differential gene expression as a function of anxiety, fearfulness or other complex traits. We selected high- and low-anxious NIH-HS rats according to the number of avoidance responses they performed in a single 50-trial session of the two-way active avoidance task. Rats were also tested in unconditioned anxiety/fearfulness tests, i.e. the elevated zero-maze and a "novel-cage activity" test. Three weeks after behavioral testing, the hippocampus was dissected and prepared for the microarray study. There appeared 29 down-regulated and 37 up-regulated SNC-related genes (fold-change>|2.19|, FDR
Databáze: OpenAIRE
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