Outer membrane Protein A plays a role in pathogenesis of Acinetobacter nosocomialis
Autor: | Sang Woo Kim, Seung Il Kim, Yoo Chul Lee, Man Hwan Oh, So Hyun Jun, Je Chul Lee, Hyejin Jeon, Kwangho Kim |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microbiology (medical) Cell Survival Virulence Factors 030106 microbiology Immunology Mutant Virulence Biology Microbiology Cell Line 03 medical and health sciences Humans Strain (chemistry) Acinetobacter Genetic Complementation Test Biofilm respiratory system biology.organism_classification 030104 developmental biology Infectious Diseases Cell culture A549 Cells Biofilms Mutation bacteria Parasitology Bacterial outer membrane Bacteria Gene Deletion Acinetobacter nosocomialis Bacterial Outer Membrane Proteins Research Paper |
Zdroj: | Virulence. 7(4) |
ISSN: | 2150-5608 |
Popis: | Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of human infections. However, the specific virulence factors of this microorganism have not yet been determined. We investigated the role of outer membrane protein A (OmpA) in the pathogenesis of A. nosocomialis. A ΔompA mutant of the A. nosocomialis ATCC 17903(T) strain was constructed using markerless gene deletion. The ΔompA mutant displayed reduced biofilm formation in polystyrene tubes and reduced adherence to A549 cells in comparison to the wild-type strain. These virulence traits of the ΔompA mutant strain were restored when the ompA gene was complemented. Cytotoxicity was not significantly different between the wild-type strain and the ΔompA mutant when A549 cells were infected with bacteria or treated with outer membrane vesicles (OMVs). However, OMVs from the wild-type strain induced cytotoxicity in HEp-2 cells, whereas OMVs from the ΔompA mutant did not induce cytotoxicity. Proteomic analysis of OMVs revealed that OmpA influenced the distribution of envelope and periplasmic proteins. Overall, this study is the first report that links OmpA to A. nosocomialis pathogenesis, and highlights OmpA as a putative target to develop anti-virulence agents or vaccines against A. nosocomialis infection. |
Databáze: | OpenAIRE |
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