Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method
| Autor: | E. Presecan, Marie-Lise Lacombe, K. Leblay, Ioan Lascu, Michel Véron |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Glycerol kinase
Biophysics Adenylate kinase Biochemistry chemistry.chemical_compound Adenosine Triphosphate Glycerol Kinase Molecular Biology chemistry.chemical_classification Oxidase test Chromatography biology Microchemistry Adenylate Kinase Deoxyguanine Nucleotides Cell Biology Nucleoside-diphosphate kinase Adenosine Diphosphate Adenosine diphosphate Kinetics Enzyme chemistry Peroxidases Nucleoside-Diphosphate Kinase biology.protein Adenosine triphosphate Oxidation-Reduction Dinucleoside Phosphates Peroxidase |
| Zdroj: | Analytical biochemistry. 209(1) |
| ISSN: | 0003-2697 |
| Popis: | A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes. |
| Databáze: | OpenAIRE |
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