Isothermal microcalorimetry for antifungal susceptibility testing of Mucorales, Fusarium spp., and Scedosporium spp
| Autor: | Jacques F. Meis, Andrej Trampuz, Ulrika Furustrand Tafin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Mucorales Isothermal microcalorimetry Posaconazole Antifungal Agents Microbial Sensitivity Tests Calorimetry Microbiology Scedosporium 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fusarium Amphotericin B medicine Humans 030212 general & internal medicine Voriconazole 0303 health sciences biology 030306 microbiology Pathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1] General Medicine biology.organism_classification Culture Media Infectious Diseases chemistry Anidulafungin Caspofungin medicine.drug |
| Zdroj: | Diagnostic Microbiology and Infectious Diseases, 73, 4, pp. 330-7 Diagnostic microbiology and infectious disease Diagnostic Microbiology and Infectious Diseases, 73, 330-7 |
| ISSN: | 0732-8893 |
| Popis: | Item does not contain fulltext We evaluated isothermal microcalorimetry for real-time susceptibility testing of non-Aspergillus molds. MIC and minimal effective concentration (MEC) values of Mucorales (n = 4), Fusarium spp. (n = 4), and Scedosporium spp. (n = 4) were determined by microbroth dilution according to the Clinical Laboratory Standard Institute M38-A2 guidelines. Heat production of molds was measured at 37 degrees C in Sabouraud dextrose broth inoculated with 2.5 x 10(4) spores/mL in the presence of amphotericin B, voriconazole, posaconazole, caspofungin, and anidulafungin. As determined by microcalorimetry, amphotericin B was the most active agent against Mucorales (MHIC 0.06-0.125 mug/mL) and Fusarium spp. (MHIC 1-4 mug/mL), whereas voriconazole was the most active agent against Scedosporium spp. (MHIC 0.25 to 8 mug/mL). The percentage of agreement (within one 2-fold dilution) between the MHIC and MIC (or MEC) was 67%, 92%, 75%, and 83% for amphotericin B, voriconazole, posaconazole, and caspofungin, respectively. Microcalorimetry provides additional information on timing of antifungal activity, enabling further investigation of drug-mold and drug-drug interaction, and optimization of antifungal treatment. 01 augustus 2012 |
| Databáze: | OpenAIRE |
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