Proteomic Analysis of the Maternal Preoptic Area in Rats
| Autor: | Katalin A. Kékesi, Edina Brigitta Udvari, Éva Hunyadi-Gulyás, Árpád Dobolyi, Katalin Völgyi, Dorina Simon |
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| Přispěvatelé: | MTA-ELTE-NAP B Molekuláris és Rendszer Neurobiológiai Kutatócsoport, MTA-ELTE Molekuláris és Rendszer Neurobiológiai Kutatócsoport, Élettani és Neurobiológiai Tanszék, Anatómiai, Szövet- és Fejlődéstani Intézet, Biológia Doktori Iskola, MTA-SE Neuromorfológiai és Neuroendokrin Kutatócsoport (2006-ig: MTA-SE Neuroendokrin Kutatócsoport), Központi Laboratóriumok, MTA-SE Neuromorfológiai Kutatócsoport (2007-től beolvadt az SE04-be), Proteomikai Csoport, MTA-ELTE Neurobiológiai Kutatócsoport (2006 végéig működött), Biokémiai Intézet |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine medicine.medical_specialty Prefrontal Cortex Biology Biochemistry Hormone receptor 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine 2-D DIGE Internal medicine Heat shock protein Neuroplasticity medicine Animals Premovement neuronal activity Electrophoresis Gel Two-Dimensional Maternal behavior Prefrontal cortex Neurons Original Paper Behavior Animal Depression Alpha-crystallin B chain—Cryab General Medicine Preoptic Area Prolactin Rats Blot Preoptic area 030104 developmental biology Endocrinology nervous system Female 030217 neurology & neurosurgery |
| Zdroj: | Neurochemical Research |
| ISSN: | 1573-6903 0364-3190 |
| Popis: | The behavior of female rats changes profoundly as they become mothers. The brain region that plays a central role in this regulation is the preoptic area, and lesions in this area eliminates maternal behaviors in rodents. The molecular background of the behavioral changes has not been established yet; therefore, in the present study, we applied proteomics to compare protein level changes associated with maternal care in the rat preoptic area. Using 2-dimensional fluorescence gel electrophoresis followed by identification of altered spots with mass spectrometry, 12 proteins were found to be significantly increased, and 6 proteins showed a significantly reduced level in mothers. These results show some similarities with a previous proteomics study of the maternal medial prefrontal cortex and genomics approaches applied to the preoptic area. Gene ontological analysis suggested that most altered proteins are involved in glucose metabolism and neuroplasticity. These proteins may support the maintenance of increased neuronal activity in the preoptic area, and morphological changes in preoptic neuronal circuits are known to take place in mothers. An increase in the level of alpha-crystallin B chain (Cryab) was confirmed by Western blotting. This small heat shock protein may also contribute to maintaining the increased activity of preoptic neurons by stabilizing protein structures. Common regulator and target analysis of the altered proteins suggested a role of prolactin in the molecular changes in the preoptic area. These results first identified the protein level changes in the maternal preoptic area. The altered proteins contribute to the maintenance of maternal behaviors and may also be relevant to postpartum depression, which can occur as a molecular level maladaptation to motherhood. |
| Databáze: | OpenAIRE |
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