Mobility Shift Affinity Capillary Electrophoresis at High Ligand Concentrations: Application to Aluminum Chlorohydrate–Protein Interactions
| Autor: | Fabien Brothier, Pavel Dubský, Hervé Cottet, Nesrine Ouadah, Jean-François Kuntz, Michal Malý, Claudine Moire |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Active ingredient
Chromatography integumentary system Chemistry General Chemical Engineering 010401 analytical chemistry 02 engineering and technology General Chemistry Electrolyte 021001 nanoscience & nanotechnology Ligand (biochemistry) 01 natural sciences 0104 chemical sciences Metal lcsh:Chemistry Electrophoresis Capillary electrophoresis Buffering agent lcsh:QD1-999 Ionic strength visual_art visual_art.visual_art_medium 0210 nano-technology |
| Zdroj: | ACS Omega, Vol 3, Iss 12, Pp 17547-17554 (2018) |
| ISSN: | 2470-1343 |
| Popis: | The active ingredients in antiperspirant products are aluminum chlorohydrates (ACHs) that, when interacting with proteins present in sweat and sweat duct walls, lead to the obstruction of the sweat ducts and thus reduce delivery of sweat at the skin surface. This study is aimed at developing a methodology based on affinity capillary electrophoresis (ACE) to obtain a quantitative ranking of the interaction between ACHs and proteins under experimental conditions close to those of industrial applications. Usually, in ACE, the metal ligand is introduced at typically μM to mM concentrations in a background electrolyte (BGE) containing a buffering agent that sets the pH and ionic strength. In this work, ACE was implemented in a range of ACH (ligand) concentrations up to 50 g/L (0.2 M in Al(H2O)6·3Cl) in the absence of other buffering agents, to mimic as much as possible the conditions encountered in the production of antiperspirant products. Under such electrophoretic conditions, the challenge is to extract qua... |
| Databáze: | OpenAIRE |
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