Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
| Autor: | Tiziana Sgamma, John Abson, Dipanwita Banik, Adrian Slater, Paul Greene, Eva Masiero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
high-resolution melt curve (HRM) analysis
Ophiopogon Liriope rbcL DNA barcoding Plant Science Biology 01 natural sciences Article Plant identification Genus lcsh:Botany medicine Ecology Evolution Behavior and Systematics Confusion Ecology Traditional medicine 010405 organic chemistry biology.organism_classification lcsh:QK1-989 0104 chemical sciences 010404 medicinal & biomolecular chemistry Real-time polymerase chain reaction Taxonomy (biology) History of use medicine.symptom |
| Zdroj: | Plants; Volume 6; Issue 4; Pages: 53 Plants Plants, Vol 6, Iss 4, p 53 (2017) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants6040053 |
| Popis: | open access article Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs. |
| Databáze: | OpenAIRE |
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