En Face Histopathologic Technic for Examining Colonic Mucosa of Resection Specimens

Autor:	H. J. R. Bussey, Stanley R. Hamilton, B C Morson
Rok vydání:	1982
Předmět:	Pathology medicine.medical_specialty Lamina propria Colon business.industry Histological Techniques Mucous membrane General Medicine Anatomy Epithelium Resection Gross examination Colonic mucosa medicine.anatomical_structure medicine Colonic mucous membrane Humans Intestinal Mucosa business
Zdroj:	American Journal of Clinical Pathology. 78:514-517
ISSN:	1943-7722 0002-9173
DOI:	10.1093/ajcp/78.4.514
Popis:	The en face histopathologic technic for examining colonic mucosa in the plane of the luminal surface is described and analyzed. Grossly normal colonic mucosa from 56 resection specimens was embedded en face and sectioned. The en face histopathologic slides showed sheets of mucosa with the crypts in cross section surrounded by lamina propria, an orientation to mucosal topography analogous to gross examination. A total of 1,931 cm2 of mucosa was embedded en face in 640 paraffin blocks, a mean of 3.0 +/- 0.8 (SD) cm2 per block. With routine processing of the same surface area, approximately 2,365 blocks (nearly four times as many as for the en face technic) would have been required. A total of 1,217 cm2 of mucosa appeared in the slides from the en face blocks, comprising 63 +/- 23% of the submitted tissue, compared with 0.3% in routine sections. Our analysis demonstrated that the en face technic is a useful tool in histopathologic studies of large areas of grossly normal colonic mucosa, particularly for quantitation and mapping of the findings.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aac85791c43cb6d879bfce4e5d6bf0a9 https://doi.org/10.1093/ajcp/78.4.514 Zobrazit plný text záznamu