Genetic mapping of a major gene affecting onion bulb fructan content
| Autor: | John McCallum, John Scheffer, Ian M. Sims, Andrew Clarke, Ruth C. Butler, Martin L. Shaw, Sjaak van Heusden, Masayoshi Shigyo, Michael J. Havey, Don W. Brash, Meeghan Pither-Joyce |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
Genetic Linkage Quantitative Trait Loci Statistics as Topic Population Locus (genetics) Breeding Biology heritability l.-aggregatum group Fructan Gene mapping traits PRI Biodiversiteit en Veredeling Onions Botany Genetics Dry matter education allium-cepa l monosomic addition lines education.field_of_study Polymorphism Genetic nonstructural carbohydrate-composition Chromosome Mapping Genetic Variation food and beverages General Medicine biology.organism_classification Major gene fistulosum l Fructans PRI Biodiversity and Breeding Phenotype soluble solids concentration Chromosomal region Allium extra chromosomes expressed sequence Agronomy and Crop Science Biotechnology |
| Zdroj: | Theoretical and Applied Genetics 112 (2006) 5 Theoretical and Applied Genetics, 112(5), 958-967 |
| ISSN: | 0040-5752 |
| DOI: | 10.1007/s00122-005-0199-5 |
| Popis: | The non-structural dry matter content of onion bulbs consists principally of fructose, glucose, sucrose and fructans. The objective of this study was to understand the genetic basis for the wide variation observed in the relative amounts of these carbohydrates. Bulb carbohydrate composition was evaluated in progeny from crosses between high dry matter storage onion varieties and sweet, low dry matter varieties. When samples were analysed on a dry weight basis, reducing sugar and fructan content exhibited high negative correlations and bimodal segregation suggestive of the action of a major gene. A polymorphic SSR marker, ACM235, was identified which exhibited strong disequilibrium with bulb fructan content in F(2:3) families from the 'W202A' x 'Texas Grano 438' mapping population evaluated in two environments. This marker was mapped to chromosome 8 in the interspecific population 'Allium cepa x A. roylei'. Mapping in the 'Colossal Grano PVP' x 'Early Longkeeper P12' F2 population showed that a dominant major gene conditioning high-fructan content lay in the same genomic region. QTL analysis of total bulb fructan content in the intraspecific mapping population 'BYG15-23' x 'AC43' using a complete molecular marker map revealed only one significant QTL in the same chromosomal region. This locus, provisionally named Frc, may account for the major phenotypic differences in bulb carbohydrate content between storage and sweet onion varieties. |
| Databáze: | OpenAIRE |
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