Regulation of cell growth and the expression of extracellular matrix proteins in colorectal adenocarcinoma: a fibroblast-tumor cell coculture model to study tumor-host interactions in vitro
| Autor: | Annalisa Siri, Stefan Berger, Antje Siegert, Carsten Denkert, Martin Köbel, Laura Borsi, Steffen Hauptmann, Stefan Ott |
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| Rok vydání: | 2003 |
| Předmět: |
Histology
Stromal cell Cell Culture Techniques Connective tissue Cell Communication Adenocarcinoma Models Biological Pathology and Forensic Medicine Transforming Growth Factor beta1 Extracellular matrix chemistry.chemical_compound Transforming Growth Factor beta Tumor Cells Cultured medicine Humans Fibroblast Extracellular Matrix Proteins biology Cell growth Tenascin C Cell Differentiation Cell Biology General Medicine Fibroblasts Coculture Techniques Fibronectin Microscopy Electron medicine.anatomical_structure chemistry Connective Tissue Chondroitin sulfate proteoglycan Immunology biology.protein Cancer research Fibroblast Growth Factor 2 Inflammation Mediators Stromal Cells Colorectal Neoplasms Cell Division |
| Zdroj: | European Journal of Cell Biology. 82:1-8 |
| ISSN: | 0171-9335 |
| DOI: | 10.1078/0171-9335-00283 |
| Popis: | Summary The production of abundant connective tissue within malignant tumors, the so-called desmoplastic stromal reaction, is a hallmark of colorectal adenocarcinomas. This stroma is produced to a large extent by myofibroblasts and contains various amounts of collagens (type I, III, and V), chondroitin sulfate proteoglycan, hyaluronic acid, fibronectin, and tenascin-C. In this study we have established a monolayer coculture model between two different colorectal adenocarcinoma cell lines (HRT-18, and CX-2) and colonic fibroblasts (CCD-18) to investigate the mechanisms regulating ( i ) the production of extracellular matrix (ECM) components, ( ii ) the induction of myofibroblastic differentiation, and ( iii ) cellular proliferation. We found that TGFβ 1 and FGF-2 stimulated ECM synthesis of fibroblasts. Myofibroblastic differentiation was stimulated by TGFβ 1 but suppressed by FGF-2. There was a mutual stimulation of proliferation between fibroblasts and carcinoma cells. The analogies with ECM components expressed in cocultures and colorectal adenocarcinoma samples suggest that the coculture model used in this study is useful to study tumor cell-fibroblast interactions. |
| Databáze: | OpenAIRE |
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