Temporal and spatial SOX9 expression patterns in the course of gonad development of the caudate amphibian Pleurodeles waltl

Autor: Dominique Chardard, Stéphane Flament, Sandra Kuntz, Amand Chesnel, Imane Al-Asaad, Hélène Dumond, Brigitte Boizet-Bonhoure
Přispěvatelé: Signalisation, Génomique et Recherche Translationnelle en Oncologie (SIGRETO), Université Henri Poincaré - Nancy 1 (UHP), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
Pleurodeles
endocrine system
Time Factors
Gonad
animal structures
media_common.quotation_subject
Molecular Sequence Data
03 medical and health sciences
0302 clinical medicine
biology.animal
Testis
Genetics
medicine
Animals
Amino Acid Sequence
Cloning
Molecular
Metamorphosis
MESH: Amino Acid Sequence Animals Cloning
Molecular Female Gene Expression Regulation
Developmental/physiology* Larva/genetics Larva/metabolism Male Molecular Sequence Data Ovary/embryology Ovary/metabolism* Phylogeny Pleurodeles/embryology* Pleurodeles/metabolism* Reverse Transcriptase Polymerase Chain Reaction SOX9 Transcription Factor/chemistry SOX9 Transcription Factor/genetics SOX9 Transcription Factor/metabolism* Sex Characteristics Testis/embryology Testis/metabolism* Time Factors
Phylogeny
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
media_common
Regulation of gene expression
Sex Characteristics
0303 health sciences
biology
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
Ovary
Gene Expression Regulation
Developmental
Vertebrate
SOX9 Transcription Factor
Sex reversal
biology.organism_classification
Cell biology
medicine.anatomical_structure
Larva
embryonic structures
Molecular Medicine
Female
Animal Science and Zoology
Amniote
Development of the gonads
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Wiley, 2011, 316B (3), pp.199-211. ⟨10.1002/jez.b.21390⟩
ISSN: 1552-5007
1552-5015
DOI: 10.1002/jez.b.21390⟩
Popis: International audience; The SOX family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Namely, SOX9 expression is conserved in vertebrate sex determination or differentiation. Nevertheless, information about caudate amphibians is lacking. In this study, we provide data on Pleurodeles waltl, a species that displays a ZZ/ZW genetic mode of sex determination and a temperature-dependent mechanism of female-to-male sex reversal. Phylogenetic analysis of SOX9 P. waltl ortholog reveals that the deduced protein segregates from the group of anuran and could be more closely related to amniote than to anamniote. However, SOX9 lacks the PQA-rich domain present in amniotes. In larvae, SOX9 is expressed in both sexes in gonad-mesonephros complexes as soon as stage 42, before gonad differentiation. At stage 54(60d) at which testis differentiation is already in progress, analyses of isolated gonads reveal a male-enriched expression of SOX9, which was quantified by real-time PCR. At the end of metamorphosis (stage 56), SOX9 shows a nuclear localization only in the testis. In adults, SOX9 is still expressed in testes and ovaries. In the ovary, SOX9 is found in oocytes from stage I to stage VI but it is never detected in the nucleus. Our results suggest that in P. waltl, like in non mammalian vertebrates, SOX9 could play a role during the late phase of gonad differentiation rather than in sex determination. Its role in germ cells of the adult ovary has still to be elucidated.
Databáze: OpenAIRE
Externí odkaz: