Can sono-photodynamic therapy enhance the antibacterial effect of curcumin against Streptococcus mutans biofilm?

Autor: Vanderlei Salvador Bagnato, Adilson César de Abreu Bernardi, Alessandra Nara de Souza Rastelli, João Felipe Besegato, Priscila Borges Gobbo de Melo
Přispěvatelé: Universidade Estadual Paulista (UNESP), University of Araraquara - Uniara, Universidade de São Paulo (USP)
Rok vydání: 2021
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1612-202X
1612-2011
DOI: 10.1088/1612-202x/ac1742
Popis: Made available in DSpace on 2022-04-29T08:33:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Sono-photodynamic therapy (SPDT) is a combined therapy which employs the use of a light source and ultrasound activation for antimicrobial purposes. This study evaluated the antibacterial effect of curcumin-mediated SPDT against Streptococcus mutans biofilm. Absorption spectra of curcumin was determined under UV-visible region (200-800 nm). The generation of reactive oxygen species was evaluated using a fluorescence probe (singlet oxygen sensor green). Minimum inhibitory and minimum bacterial concentrations were determined. S. mutans biofilm was cultured and treated according to the groups as follows: L - PS - U- (negative control), chlorhexidine (positive control), L + PS + U- (aPDT groups), L - PS + U+ (SDT groups), L + PS + U+ (SPDT groups). Before irradiation, the biofilms were incubated for 5 min and irradiated by blue light emitting-diode at 15 J cm-2. For the dark toxicity, the groups were exposed to the same conditions, but no light was used. After treatments, counting of colonies forming units was performed. Confocal microscopy images were obtained. Data were statistically analyzed by ANOVA and Tukey's test (p 0.05). Curcumin under 80 µM showed higher absorption than 40 µM. For the generation of reactive species, antimicrobial photodynamic therapy and SPDT exhibited similar behavior. Minimum inhibitory concentration and minimum bactericidal concentration were 40 and 80 µM, respectively. Curcumin under 40 and 80 µM showed no dark toxicity. SPDT showed better results than isolated therapies, since significant reduction (3.8 log10; p 0.001) over the cell viability was observed. This result was confirmed by confocal images. Curcumin showed promising results as a photosensitizer for SPDT. Moreover, curcumin-mediated SPDT exhibited enhanced antibacterial effects and may be an alternative therapy to control the oral biofilm. Department of Restorative Dentistry School of Dentistry São Paulo State University - UNESP Araraquara Department of Biology and Health Sciences University of Araraquara - Uniara Physics Institute of São Carlos-IFSC University of São Paulo - Usp Department of Restorative Dentistry School of Dentistry São Paulo State University - UNESP Araraquara
Databáze: OpenAIRE