Lateral diffusion of cholesterol and dimyristoylphosphatidylcholine in a lipid bilayer measured by pulsed field gradient NMR spectroscopy
| Autor: | P.W. Westerman, Göran Lindblom, Greger Orädd |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Magnetic Resonance Spectroscopy
Chemistry Diffusion Lipid Bilayers Analytical chemistry technology industry and agriculture Biophysics Temperature Nuclear magnetic resonance spectroscopy Activation energy Lipids Biophysical Phenomena Cholesterol Lamellar phase Phase (matter) Lamellar structure lipids (amino acids peptides and proteins) Pulsed field gradient Lipid bilayer Dimyristoylphosphatidylcholine Research Article |
| Popis: | The pulsed field gradient NMR method for measuring self-diffusion has been used for a direct determination of the lateral diffusion coefficient of cholesterol, fluorine labeled at the 6-position, for an oriented lamellar liquid-crystalline phase of dimyristoylphosphatidylcholine (DMPC)/cholesterol/water. It is found that the diffusion coefficients of DMPC and cholesterol are equal over a large temperature interval. The apparent energy of activation for the diffusion process (58 kJ/mol) is about the same as for a lamellar phase of DMPC/water, whereas the phospholipid lateral diffusion coefficient is approximately four times smaller in the presence of cholesterol. |
| Databáze: | OpenAIRE |
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