Identification of a cell-penetrating peptide domain from human beta-defensin 3 and characterization of its anti-inflammatory activity
| Autor: | Jue Yeon Lee, Jeong Hwa Kim, Hyun-Jung Park, Jin Sook Suh, Yoon Jeong Park, Chong Pyoung Chung, Jung Min Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Male beta-Defensins Lipopolysaccharide Cell human beta-defensin 3 cell-penetrating peptide anti-inflammatoryactivity lipopolysaccharide NF-kappa B canonical pathway Anti-Inflammatory Agents Pharmaceutical Science Nitric Oxide Synthase Type II Cell-Penetrating Peptides chemistry.chemical_compound Mice International Journal of Nanomedicine NF-κB canonical pathway Drug Discovery Leukocytes Phosphorylation anti-inflammatory activity Original Research NF-kappa B General Medicine Cell biology medicine.anatomical_structure Cytokines Tumor necrosis factor alpha medicine.symptom Bacterial outer membrane Cell Survival Biophysics Mice Nude Bioengineering Inflammation Biology Nitric Oxide Biomaterials medicine Animals Humans Rats Wistar Innate immune system Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Organic Chemistry NFKB1 Immunity Innate Protein Structure Tertiary Rats Beta defensin chemistry Microscopy Fluorescence Immunology |
| Zdroj: | International Journal of Nanomedicine INTERNATIONAL JOURNAL OF NANOMEDICINE(10) |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Jue Yeon Lee,1,* Jin Sook Suh,2,* Jung Min Kim,1 Jeong Hwa Kim,1 Hyun Jung Park,1 Yoon Jeong Park,1,2 Chong Pyoung Chung1 1Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), Chungcheongbuk-do, Republic of Korea; 2Dental Regenerative Biotechnology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea *These authors contributed equally tothis work Abstract: Human beta-defensins (hBDs) are crucial factors of intrinsic immunity that function in the immunologic response to a variety of invading enveloped viruses, bacteria, and fungi. hBDs can cause membrane depolarization and cell lysis due to their highly cationic nature. These molecules participate in antimicrobial defenses and the control of adaptive and innate immunity in every mammalian species and are produced by various cell types. The C-terminal 15-mer peptide within hBD3, designated as hBD3-3, was selected for study due to its cell- and skin-penetrating activity, which can induce anti-inflammatory activity in lipopolysaccharide-treated RAW 264.7 macrophages. hBD3-3 penetrated both the outer membrane of the cells and mouse skin within a short treatment period. Two other peptide fragments showed poorer penetration activity compared to hBD3-3. hBD3-3 inhibited the lipopolysaccharide-induced production of inducible nitric oxide synthase, nitric oxide, and secretory cytokines, such as interleukin-6 and tumor necrosis factor in a concentration-dependent manner. Moreover, hBD3-3 reduced the interstitial infiltration of polymorphonuclear leukocytes in a lung inflammation model. Further investigation also revealed that hBD3-3 downregulated nuclear factor kappa B-dependent inflammation by directly suppressing the degradation of phosphorylated-IκBα and by downregulating active nuclear factor kappa B p65. Our findings indicate that hBD3-3 may be conjugated with drugs of interest to ensure their proper translocation to sites, such as the cytoplasm or nucleus, as hBD3-3 has the ability to be used as a carrier, and suggest a potential approach to effectively treat inflammatory diseases. Keywords: human beta-defensin 3, cell-penetrating peptide, anti-inflammatory activity, lipopolysaccharide, NF-κB canonical pathway |
| Databáze: | OpenAIRE |
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