Deriving Functional Astrocytes from Mouse Embryonic Stem Cells with a Fast and Efficient Protocol
Autor: | Evangelos Delivopoulos, Slawomir J. Nasuto, Deppo S. Juneja |
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Rok vydání: | 2019 |
Předmět: |
Cellular differentiation
Central nervous system Population Cell Culture Techniques Synaptogenesis S100 Calcium Binding Protein beta Subunit Biology Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Glial Fibrillary Acidic Protein medicine Animals education 030304 developmental biology Aquaporin 4 Oxidoreductases Acting on CH-NH Group Donors 0303 health sciences education.field_of_study Cell Differentiation Mouse Embryonic Stem Cells Phenotype Embryonic stem cell medicine.anatomical_structure Astrocytes Stem cell Neuroscience 030217 neurology & neurosurgery |
Zdroj: | EMBC |
Popis: | A growing number of studies highlight the structural and functional diversity of astrocytes throughout the central nervous system. These cells are now seen as heterogeneous as neurons and are implicated in a number of neurological and psychiatric diseases. Efficient generation of diverse subtypes of astrocytes can be a useful tool in investigating synaptogenesis and patterns of activity in developing neural networks. In this study, we developed a protocol for the fast and efficient differentiation of astrocytes from mouse embryonic stem cells, as evidenced by the upregulation of genes related to astrocytic development (Gfap, Aldh1l1). Generated astrocytes exhibit phenotypic diversity, which is demonstrated by the variant expression of markers such as GFAP, ALDH1L1, AQP4 and S100β, amongst subgroups within the same cell population. In addition, astrocytes exhibited differential calcium transients upon stimulation with ATP. Our protocol will facilitate investigations, regarding the involvement of astrocytes in the structural and functional connectivity of neural networks. |
Databáze: | OpenAIRE |
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