A novel and simple method for endotracheal intubation of mice
| Autor: | E N Spoelstra, R. Remie, Ben H.C. Westerink, C Ince, Linda A. Brouwer, V M Emons, Anneke Koeman, M.J.A. van Luyn |
|---|---|
| Přispěvatelé: | Biomonitoring and Sensoring, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Biomedical Engineering and Physics, Translational Physiology, Anesthesiology |
| Rok vydání: | 2007 |
| Předmět: |
Male
Larynx Epiglottis medicine.medical_specialty medicine.medical_treatment inhalation anaesthetics mechanical ventilation Lung injury MOUSE Mice Intubation Intratracheal medicine Animals Intubation general anaesthesia General anaesthesia Lung endotracheal intubation Mechanical ventilation General Veterinary Inhalation laryngoscopic blade business.industry Respiration Artificial Surgery Mice Inbred C57BL Intubation procedure medicine.anatomical_structure INTRATRACHEAL INSTILLATION Anesthesia Animal Science and Zoology Blood Gas Analysis business LUNG INJURY |
| Zdroj: | Laboratory Animals, 41(1), 128-135 Laboratory animals, 41(1), 128-135. SAGE Publications Ltd |
| ISSN: | 1758-1117 0023-6772 |
| Popis: | Endotracheal intubation in mice is necessary for experiments involving intratracheal instillation of various substances, repeated pulmonary function assessments and mechanical ventilation. Previously described methods for endotracheal intubation in mice require the use of injection anaesthesia to immobilize the animal during the intubation procedure or the use of a volatile anaesthetic prior to intubation for immobilization. With these methods, the control of anaesthetic depth during the intubation procedure is absent. We describe a method for simple and rapid intratracheal intubation in mice for mechanical ventilation, using a self-built plastic support to facilitate the intubation procedure. General anaesthesia is maintained by means of inhalation through a non-rebreathing circuit connected to the plastic support. This set-up gives the operator control of anaesthetic depth and sufficient time to perform the intubation procedure. A purpose-made laryngoscopic blade is used to facilitate the intubation tube entering the trachea. The blade of the purpose-made laryngoscope is constructed as a retraction guide and is curved for easy handling. Under direct vision, the epiglottis is gently lifted by the laryngoscopic blade while the intubation tube is pushed into the trachea. Following this novel intubation technique, we were able to mechanically ventilate mice for at least 2 h without severely disturbing blood gases. Histological evaluation of the lungs and microscopic evaluation of the trachea and larynx showed no signs of trauma related to the intubation technique or mechanical ventilation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|