Genome-Wide Association Study (GWAS) for Examining the Genomics Controlling Prickle Production in Red Raspberry (Rubus idaeus L.)
Autor: | Courtney A. Weber, Archana Khadgi |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Rubus Population gene s black raspberry Single-nucleotide polymorphism Genomics Genome-wide association study 01 natural sciences gene H lcsh:Agriculture 03 medical and health sciences Black raspberry education Gene 030304 developmental biology Genetics 0303 health sciences education.field_of_study spinelessness biology lcsh:S food and beverages thornless biology.organism_classification spine free Blowing a raspberry Agronomy and Crop Science blackberry 010606 plant biology & botany |
Zdroj: | Agronomy, Vol 11, Iss 27, p 27 (2021) Agronomy Volume 11 Issue 1 |
ISSN: | 2073-4395 |
Popis: | Red raspberry (Rubus idaeus L.) is an expanding high-value berry crop worldwide. The presence of prickles, outgrowths of epidermal tissues lacking vasculature, on the canes, petioles, and undersides of leaves complicates both field management and harvest. The utilization of cultivars with fewer prickles or prickle-free canes simplifies production. A previously generated population segregating for prickles utilizing the s locus between the prickle-free cultivar Joan J (ss) and the prickled cultivar Caroline (Ss) was analyzed to identify the genomic region associated with prickle development in red raspberry. Genotype by sequencing (GBS) was combined with a genome-wide association study (GWAS) using fixed and random model circulating probability unification (FarmCPU) to analyze 8474 single nucleotide polymorphisms (SNPs) and identify significant markers associated with the prickle-free trait. A total of four SNPs were identified on chromosome 4 that were associated with the phenotype and were located near or in annotated genes. This study demonstrates how association genetics can be used to decipher the genetic control of important horticultural traits in Rubus, and provides valuable information about the genomic region and potential genes underlying the prickle-free trait. |
Databáze: | OpenAIRE |
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