A combined method for correlative 3D imaging of biological samples from macro to nano scale
| Autor: | Nicole Izykowski, Manuela Kellner, Christoph Wrede, Roman Grothausmann, Mark P. Kühnel, Matthias Ochs, Lars Knudsen, Georgios C. Antonopoulos, Raoul-Amadeus Lorbeer, Heiko Meyer, Tammo Ripken, Marko Heidrich, Danny Jonigk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Correlative Materials science multimodal imaging tomography Multimodal Imaging Article lung law.invention 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine law Pathology medicine Animals animal rat Sample preparation procedures Optical tomography Lung Tomography Nanoscopic scale Dewey Decimal Classification::500 | Naturwissenschaften Interconnection Multidisciplinary medicine.diagnostic_test three dimensional imaging Laser Dewey Decimal Classification::600 | Technik Rats 030104 developmental biology Transmission electron microscopy ddc:500 ddc:600 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports 6 (2016) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep35606 |
| Popis: | Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques. |
| Databáze: | OpenAIRE |
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