Ketamine ameliorates ischemia–reperfusion injury after liver autotransplantation by suppressing activation of Kupffer cells in rats
| Autor: | Xue-Min Song, Chaoliang Tang, Peipei Guo, Zongze Zhang, Cheng-yao Wang, Jin Wang, Yanlin Wang, Xinyi Li, Zhao Jin, Huisheng Wu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Kupffer Cells Physiology medicine.medical_treatment Ischemia Apoptosis Inflammation Liver transplantation Pharmacology Transplantation Autologous Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Physiology (medical) medicine Animals Ketamine RNA Messenger business.industry I r injury NF-kappa B General Medicine medicine.disease Autotransplantation Liver Transplantation Rats 030104 developmental biology Cytoprotection Reperfusion Injury 030220 oncology & carcinogenesis Rat liver Hepatocytes medicine.symptom business Reperfusion injury Signal Transduction medicine.drug |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 96:886-892 |
| ISSN: | 1205-7541 0008-4212 |
| Popis: | This study aimed to investigate the protective effects of ketamine against hepatic ischemia–reperfusion (I/R) injury by suppressing activation of Kupffer cells (KCs) in rat liver autotransplantation. Male Sprague-Dawley rats were randomized into 3 groups (n = 10 each). Group I, the sham group, received saline. Group II received saline and underwent orthotopic liver autotransplantation (OLAT). Group III received 10 mg/kg ketamine and underwent OLAT. Blood samples were obtained at 3, 6, 12, and 24 h after I/R, and following ALT, AST, LDH, IL-6, TNF-α, IL-1β, and IL-10 in serum were detected. Model rats were sacrificed at the indicated time points and the graft liver tissues were evaluated histologically. KCs were isolated from rat liver tissues, and inflammatory products and proteins of NF-κB signaling pathway were detected using quantitative RT–PCR and Western blotting. Our results showed that ketamine significantly decreased ALT, AST, LDH, IL-6, TNF-α, and IL-1β levels and increased IL-10 level. Furthermore, ketamine alleviated the histopathology changes, by less KC infiltration and lower hepatocyte apoptosis. Moreover, activity of NF-κB signaling pathway in KCs was suppressed. In addition, production of pro- and anti-inflammatory factors is consistent with the results in tissues. Ketamine ameliorated I/R injury after liver transplantation by suppressing activation of KCs in rats. |
| Databáze: | OpenAIRE |
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