Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform
| Autor: | Christian Plank, Alvaro J. Conde, Lucia Policastro, Osvaldo L. Podhajcer, Rossana E. Madrid, Belén Cerda, J.M. Cabaleiro, Olga Mykhaylyk, Milena Batalla |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Ethanol Continuous flow Microfluidics Biomedical Engineering Nanoparticle Bioengineering Laminar flow Nanotechnology Equipment Design General Chemistry Microfluidic Analytical Techniques Magnetic field gradient Lipids Biochemistry Nephelometry and Turbidimetry Magnet Liposomes Hydrodynamics Magnets Nanoparticles Nanomedicine Computer Simulation Biomedical engineering |
| Zdroj: | Lab Chip. 14:4506-4512 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | We present a low-cost, portable microfluidic platform that uses laminated polymethylmethacrylate chips, peristaltic micropumps and LEGO® Mindstorms components for the generation of magnetoliposomes that does not require extrusion steps. Mixtures of lipids reconstituted in ethanol and an aqueous phase were injected independently in order to generate a combination of laminar flows in such a way that we could effectively achieve four hydrodynamic focused nanovesicle generation streams. Monodisperse magnetoliposomes with characteristics comparable to those obtained by traditional methods have been obtained. The magnetoliposomes are responsive to external magnetic field gradients, a result that suggests that the nanovesicles can be used in research and applications in nanomedicine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|