Whole Genome Analysis of a Wine Yeast Strain
| Autor: | Kurt Fellenberg, Rosario Gil, Nicole Hauser, Sonja Bastuck, Jörg D. Hoheisel, José E. Pérez-Ortín |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Genetics
Wine Article Subject lcsh:QH426-470 Strain (biology) Saccharomyces cerevisiae food and beverages Biology biology.organism_classification Genome Phenotype Yeast in winemaking lcsh:Genetics lcsh:Biology (General) lcsh:Q Copy-number variation lcsh:Science Molecular Biology Gene lcsh:QH301-705.5 Biotechnology Research Article |
| Zdroj: | Comparative and Functional Genomics, Vol 2, Iss 2, Pp 69-79 (2001) Comparative and Functional Genomics |
| ISSN: | 1532-6268 1531-6912 |
| Popis: | Saccharomyces cerevisiaestrains frequently exhibit rather specific phenotypic features needed for adaptation to a special environment. Wine yeast strains are able to ferment musts, for example, while other industrial or laboratory strains fail to do so. The genetic differences that characterize wine yeast strains are poorly understood, however. As a first search of genetic differences between wine and laboratory strains, we performed DNA-array analyses on the typical wine yeast strain T73 and the standard laboratory background in S288c. Our analysis shows that even under normal conditions, logarithmic growth in YPD medium, the two strains have expression patterns that differ significantly in more than 40 genes. Subsequent studies indicated that these differences correlate with small changes in promoter regions or variations in gene copy number. Blotting copy numbers vs. transcript levels produced patterns, which were specific for the individual strains and could be used for a characterization of unknown samples. |
| Databáze: | OpenAIRE |
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