Effects of growth hormone and cortisol administration on plasma insulin-like growth factor binding proteins in juveniles of three subspecies of masu salmon (Oncorhynchus masou)
| Autor: | Takuro Nakajima, Yuki Ugachi, Hisashi Kawai, Munetaka Shimizu, Ginnosuke Yamaguchi, Shiori Habara, Shotaro Suzuki |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
0301 basic medicine endocrine system medicine.medical_specialty Hydrocortisone Oncorhynchus Insulin-like growth factor binding protein subtypes Physiology medicine.medical_treatment Blotting Western 030209 endocrinology & metabolism Subspecies Growth hormone action Growth hormone Biochemistry DNA-binding protein 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Species Specificity Internal medicine medicine Animals Protein Isoforms Inducer Hormonal control Insulin-Like Growth Factor I Acute stress Molecular Biology biology Growth factor Ligand blotting biology.organism_classification Insulin-Like Growth Factor Binding Protein 1 030104 developmental biology Endocrinology Growth Hormone Oncorhynchus masou subspecies-complex hormones hormone substitutes and hormone antagonists |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 251:110821 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2020.110821 |
| Popis: | In this study, we examined the effects of porcine growth hormone (GH) and cortisol on plasma insulin-like growth factor binding proteins (IGFBPs) in juveniles of three subspecies of Oncorhynchus masou (masu, amago, and Biwa salmon). Ligand blotting using digoxigenin-labeled human IGF-I was used to detect and semi-quantify three major circulating IGFBP bands at 41, 28, and 22 kDa, corresponding to IGFBP-2b, −1a, and -1b, respectively. GH increased plasma IGFBP-2b concentration in masu and Biwa salmon but suppressed it in amago salmon. Plasma IGFBP-2b levels were increased by cortisol in the three subspecies. Cortisol induced plasma IGFBP-1a in the three subspecies, whereas GH had a suppressive effect in masu and Biwa salmon. Sham and cortisol injections increased plasma IGFBP-1b levels after 1 day in masu and amago salmon, suggesting that IGFBP-1b is induced following exposure to stressors via cortisol. Increased IGFBP-1b levels were restored to basal levels when co-injected with GH in Biwa salmon, and the same trend was seen in masu and amago salmon. However, the suppressive effect of GH disappeared 2 days after injection in the three subspecies. Despite some differences among subspecies, the findings suggest that cortisol is a primary inducer of plasma IGFBP-1b; however, GH counteracts it in the short term. Therefore, GH has the potential to modulate the degree of increase in circulating IGFBP-1b levels during acute stress. |
| Databáze: | OpenAIRE |
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