Slipped DNA structures within the enhancer region of the Moloney murine leukemia virus
| Autor: | Jessica C. Hall, Ruth M. Ruprecht, Miguel A. Gama Sosa |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Genes Viral viruses Molecular Sequence Data Biophysics Biochemistry Virus chemistry.chemical_compound Plasmid Murine leukemia virus Escherichia coli Direct repeat Cloning Molecular Enhancer Molecular Biology Nuclease biology Base Sequence Cell Biology biology.organism_classification Virology Molecular biology Long terminal repeat Enhancer Elements Genetic chemistry Genes DNA Viral biology.protein Nucleic Acid Conformation Moloney murine leukemia virus DNA Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 156(1) |
| ISSN: | 0006-291X |
| Popis: | We have examined the Sl nuclease sensitivity of supercoiled plasmids harboring the Moloney Murine Leukemia Virus (MoMuLV) long terminal repeat (LTR). S1 sensitivity was found within the LTR enhancer direct repeats. Transformation of E. coli DH5 cells with a construct containing most of the MoMuLV LTR yielded the precise deletion of one direct repeat and loss of S1 sensitivity. The dependence of S1 sensitivity on the presence of both direct repeats, together with the exact excision of one direct repeat by E. coli , suggests the presence of slipped DNA within the enhancer. Such structures may represent targets for effector proteins which mediate vital functions during viral propagation. |
| Databáze: | OpenAIRE |
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