Frequencies at three polymorphic sites of interleukin-10 gene promoter in Brazilian renal recipients
| Autor: | Maria da Graça Bicalho, Ricardo Benvenutti, F.L.C Contieri, A Plothow |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Linkage disequilibrium Adolescent Genotype Population Biology Polymorphism Single Nucleotide White People Polymorphism (computer science) Humans Allele Child Promoter Regions Genetic education Gene Genetics Transplantation education.field_of_study Base Sequence Haplotype Middle Aged Kidney Transplantation Interleukin-10 Surgery Brazil |
| Zdroj: | Transplantation Proceedings. 35:2908-2910 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2003.10.013 |
| Popis: | We genotyped by PCR-RFLP 141 renal patients (77 men and 64 women with ages between 12 and 58, predominantly Caucasians) for allelic variants of three polymorphic sites of the interleukin-10 gene promoter, previously showed to be associated with different production of IL-10 cytokine: -1082 (G/A), -819 (C/T), and -592 (C/A). These polymorphisms may confer flexibility in the common immune responses and influence the outcome of allo-responses after transplantation. Our aim was to determine the frequencies of three functional polymorphic sites -1082, -819, and -592 of the interleukin-10 gene promoter in renal recipients of Curitiba, Paraná. Paraná State is located in southern Brazil, and its capital is Curitiba with approximately 1.5 million inhabitants. Genotypes were classified as follows: "low" IL-10 producer genotypes (ATA/ATA, ACC/ATA, ACC/ACC), "intermediate" genotypes (GCC/ACC, GCC/ATA), and "high" IL-10 producer genotype (GCC/GCC). In our population we observed linkage disequilibrium between alleles -819C and -592C and this haplotypic combination was more frequent (65%) than -819T and -592A. We found significantly reduced frequency of the genotype and haplotype responsible for high production of interleukin-10, maybe because we have a selected group (only renal patients) or maybe because Brazilian people are very heterogeneous (miscegenational), which may differ from other groups. |
| Databáze: | OpenAIRE |
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