VEGF enhance cortical newborn neurons and their neurite development in adult rat brain after cerebral ischemia
| Autor: | Yong-Quan Wang, Xiao-Yuan Feng, Hui-Ru Cui, Shanzheng Yang, Hua-Ping Sun, Mei-Hong Qiu, Feng-Yan Sun |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A DNA Complementary Neurite Angiogenesis Antimetabolites Blotting Western Fluorescent Antibody Technique Transfection Brain Ischemia Cell Line Andrology Brain ischemia Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Neurites Animals Humans Cerebral Cortex Neurons Microscopy Confocal Chemistry Neurogenesis Infarction Middle Cerebral Artery Cell Biology Anatomy medicine.disease Immunohistochemistry Magnetic Resonance Imaging Rats medicine.anatomical_structure nervous system Animals Newborn Bromodeoxyuridine Cerebral cortex GABAergic Plasmids |
| Zdroj: | Neurochemistry international. 55(7) |
| ISSN: | 1872-9754 |
| Popis: | To study the effect of VEGF overexpression on development of cortical newborn neurons in the brains after stroke, we injected human VEGF(165)-expressive plasmids (phVEGF) into the lateral ventricle of rat brains with a transient middle cerebral artery occlusion (MCAO). An injection of phVEGF significantly promoted angiogenesis (BrdU(+)-von Willebrand's factor(+)) and reduced infarct volume in the rat brain after MCAO. Single labeling of 5'-bromodeoxyuridine (BrdU) and double staining of BrdU with lineage-specific neuronal markers were used to indicate the proliferated cells and maturation of newborn neurons in the brain section of rats at 2, 4, and 8 weeks after MCAO. The results showed that BrdU positive (BrdU(+)) cells existed in ipsilateral frontal cortex within 8 weeks after MCAO and reached the maximum at 2 weeks of reperfusion. The phVEGF treatment significantly increased BrdU(+) cells compared with the control plasmid (pEGFP) injection. Cortical neurogenesis was indicated by the presence of newborn immature (BrdU(+)-Tuj1(+)), newborn mature (BrdU(+)-MAP-2(+)), and newborn GABAergic (BrdU(+)-GAD67(+)) neurons. All these neurons declined within 8 weeks after MCAO in the controls. Injection of phVEGF significantly increased BrdU(+)-Tuj1(+) neurons at 2 weeks, and BrdU(+)-MAP-2(+) neurons and BrdU(+)-GAD67(+) neurons at 4 and 8 weeks, respectively after MCAO. Moreover, phVEGF treatment significantly increased neurite length and branch numbers of BrdU(+)-MAP-2(+) newborn neurons compared with pEGFP treatment. These results demonstrate that VEGF enhances maturation of stroke-induced cortical neurogenesis and dendritic formation of newborn neurons in adult mammalian brains. |
| Databáze: | OpenAIRE |
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