In Vivo Distribution of Poly(ethylene glycol) Functionalized Iron Oxide Nanoclusters: An Ultrastructural Study
| Autor: | Maria Suciu, Loredana Olar, Lucian Barbu-Tudoran, Claudiu Mirescu, Rǎzvan Ştefan, Alexandra Ciorîță, Sergiu Macavei, Cristian Leoștean, Septimiu-Cassian Tripon, Izabell Craciunescu |
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| Rok vydání: | 2021 |
| Předmět: |
magnetic nanoparticles
General Chemical Engineering Iron oxide Spleen Urine liver Article in vivo tracking law.invention Nanoclusters lung chemistry.chemical_compound law In vivo medicine General Materials Science QD1-999 Kidney SPIONs-PEG electron microscopy Chemistry medicine.anatomical_structure Ultrastructure Electron microscope Nuclear chemistry |
| Zdroj: | Nanomaterials Volume 11 Issue 9 Nanomaterials, Vol 11, Iss 2184, p 2184 (2021) |
| ISSN: | 2079-4991 |
| Popis: | The in vivo distribution of 50 nm clusters of polyethylene glycol-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-PEG) was conducted in this study. SPIONs-PEG were synthesized de novo, and their structure and paramagnetic behaviors were analyzed by specific methods (TEM, DLS, XRD, VSM). Wistar rats were treated with 10 mg Fe/kg body weight SPIONs-PEG and their organs and blood were examined at two intervals for short-term (15, 30, 60, 180 min) and long-term (6, 12, 24 h) exposure evaluation. Most exposed organs were investigated through light and transmission electron microscopy, and blood and urine samples were examined through fluorescence spectrophotometry. SPIONs-PEG clusters entered the bloodstream after intraperitoneal and intravenous administrations and ended up in the urine, with the highest clearance at 12 h. The skin and spleen were within normal histological parameters, while the liver, kidney, brain, and lungs showed signs of transient local anoxia or other transient pathological affections. This study shows that once internalized, the synthesized SPIONs-PEG disperse well through the bloodstream with minor to nil induced tissue damage, are biocompatible, have good clearance, and are suited for biomedical applications. |
| Databáze: | OpenAIRE |
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