An optimised method for the rapid analysis of condensed phosphates in fishery and processed marine food products using ion chromatography and microwave sample processing
| Autor: | Miri Lee, Kyung Min Lim, Dal Woong Choi, Ye Ji Koo, Dae Yong Jang, Eun Chul Pack, Seung Ha Lee, Hyung Soo Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Hydrolases
Health Toxicology and Mutagenesis Sample processing Ion chromatography Fisheries Toxicology Sensitivity and Specificity 01 natural sciences Phosphates chemistry.chemical_compound Hydrolysis 0404 agricultural biotechnology Limit of Detection Animals Microwaves Solid Phase Extraction 010401 analytical chemistry Public Health Environmental and Occupational Health 04 agricultural and veterinary sciences General Chemistry General Medicine Repeatability Chromatography Ion Exchange Phosphate 040401 food science 0104 chemical sciences Fishery Seafood chemistry Food products Retention time Microwave Food Science |
| Zdroj: | Food Additives & Contaminants: Part A. 37:205-215 |
| ISSN: | 1944-0057 1944-0049 |
| DOI: | 10.1080/19440049.2019.1693634 |
| Popis: | Condensed phosphates are deliberately added to the fishery and processed marine food products on purpose to increase the weight of products. However, excessive intake overwhelming bodily homoeostasis can result in phosphate toxicity such as mineral and bone disorders, associated with chronic kidney diseases, and cardiovascular events. Rapid analysis for condensed phosphates is required to detect the illegal adulteration of processed marine products. We optimised an analytical method using ion chromatography for the rapid and selective detection of condensed phosphates in various kinds of fishery and processed marine products. We compared the performance of three columns (IonPac AS11, AS11-HC, and AS16) for the detection of condensed phosphates with respect to time of analysis and sensitivity. The IonPac AS11 column exhibited the shortest retention time for the major condensed phosphates (pyro-, tri-, and trimetaphosphate), the highest sensitivity for trimetaphosphate, and good repeatability and precision. Microwave and boiling processing were examined as methods to prevent hydrolysis of condensed phosphates into orthophosphate, which is critical in distinguishing intentionally added condensed phosphates from naturally occurring orthophosphate. Microwave treatment was determined to be the more effective method to suppress hydrolysis of condensed phosphates to orthophosphate. Furthermore, microwave processing could be used for thawing the frozen samples, saving extra effort and time. We confirmed that the method exhibits good recovery (80% or more) and precision (%RSD < 10%) for samples with various matrices. With the method, 14 kinds of fishery and processed marine food products were successfully analysed for condensed phosphates. Especially, we identified that phosphate levels in the processed shrimp and dried shredded squid samples exceeded the maximum allowable levels specified in the CODEX standard. We believe that our method would be useful for the rapid analysis of condensed phosphates in various types of fishery and processed marine food products. |
| Databáze: | OpenAIRE |
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