Effect of aldosterone and glycyrrhetinic acid on the protein expression of PAI-1 and p22(phox) in human mononuclear leukocytes
| Autor: | Calo', Lorenzo, Zaghetto, F, Pagnin, Elisa, Davis, Pa, DE MOZZI, P, Sartorato, Paola, Martire, G, Fiore, Cristina, Armanini, Decio, Calo', Pa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male medicine.medical_specialty Aldosterone Glycyrrhetinic acid Mononuclear leukocytes medicine.drug_class Endocrinology Diabetes and Metabolism Clinical Biochemistry medicine.disease_cause Biochemistry chemistry.chemical_compound Endocrinology Internal medicine Canrenone Plasminogen Activator Inhibitor 1 medicine Humans Receptor Enoxolone Mineralocorticoid Receptor Antagonists biology Dose-Response Relationship Drug Biochemistry (medical) NADPH Dehydrogenase Membrane Transport Proteins NADPH Oxidases Drug Synergism medicine.disease Phosphoproteins Hyperaldosteronism chemistry Mineralocorticoid biology.protein Leukocytes Mononuclear Glycyrrhetinic Acid Female P22phox Oxidative stress medicine.drug |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 89(4) |
| ISSN: | 0021-972X |
| Popis: | Aldosterone excess can produce heart and kidney fibrosis, which seem to be related to a direct effect of aldosterone at the level of specific receptors. We report a direct, mineralocorticoid-mediated effect on the protein expression of two markers of oxidative stress after incubation of mononuclear leukocytes with 1 × 10−8m aldosterone (p22phox/β-actin = 1.38 ± 0.05 and PAI-1/β-actin = 1.80 ± 0.05). The same effect was also found with 3 × 10−5m glycyrrhetinic acid, the principal constituent of licorice root (p22phox/β-actin = 1.37 ± 0.97 and PAI-1/β-actin = 1.80 ± 0.04). The effect of both aldosterone and glycyrrhetinic acid is blocked by incubation with added 1 × 10−6m of receptor-antagonist canrenone. Canrenone alone did not show any effect. PAI-1 related protein was also found using 4 × 10−9m aldosterone. Incubations with 1 × 10−9m for 3 hours as well as 1 × 10−8m aldosterone for 5, 10, and 20 minutes were ineffective for both proteins. These data support the previous finding of an involvement of mononuclear leukocytes in the pathogenesis of the oxidative stress induced by hyperaldosteronism. In addition, the results confirm our previous data on a direct effect of glycyrrhetinic acid at the level of mineralocorticoid receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|