C/EBP and C-Myb sites are important for the functional activity of the human myeloperoxidase upstream enhancer
| Autor: | Congjun Yao, Kimberly Works, Zhenyu Qin, Garth E. Austin, Andrew N. Young |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Biophysics
Biology Biochemistry Proto-Oncogene Proteins c-myb Genes Reporter Transcription (biology) Cell Line Tumor Deoxyribonuclease I Humans MYB Protein Footprinting Enhancer Molecular Biology Peroxidase Regulation of gene expression Binding Sites Ccaat-enhancer-binding proteins Cell Biology Molecular biology Upstream Enhancer Enhancer Elements Genetic Gene Expression Regulation Myeloperoxidase CCAAT-Enhancer-Binding Proteins biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 371:309-314 |
| ISSN: | 0006-291X |
| Popis: | Myeloperoxidase (MPO), an enzyme active against bacterial and fungal infections, is expressed specifically in myeloblasts and promyelocytes and minimal in other cell types. We recently identified and partially characterized an upstream enhancer located between -4100 and -3844 bp of the MPO gene. We showed that an AML1 site contributes to enhancer activity and specificity. We now demonstrate three additional footprints within the MPO enhancer and provide evidence that C/EBP and c-Myb sites contribute to its functional, tissue-specific activity. This distal enhancer appears to play an important role in the control of MPO transcription during differentiation of myeloid cells. |
| Databáze: | OpenAIRE |
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