Spontaneous and radiation-induced chromosomal instability and persistence of chromosome aberrations after radiotherapy in lymphocytes from prostate cancer patients

Autor: Andrea Hille, Hana Hofman-Hüther, Barbara Wilken, Patricia Virsik, Elna Kühnle, Margret Rave-Fränk, Heinz Schmidberger
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Male
Pathology
medicine.medical_specialty
medicine.medical_treatment
Biophysics
Biology
03 medical and health sciences
Dicentric chromosome
Prostate cancer
0302 clinical medicine
Environmental Science(all)
Prostate
Chromosome instability
Chromosomal Instability
Physics
Ecosystems
Environmental Physics
Environmental Monitoring/Analysis
Biophysics and Biological Physics
Effects of Radiation/Radiation Protection
Carcinoma
medicine
Humans
Genetic Predisposition to Disease
Lymphocytes
Radiation Injuries
030304 developmental biology
General Environmental Science
Aged
Aged
80 and over
0303 health sciences
Original Paper
Radiation
Chromosome
Prostatic Neoplasms
Middle Aged
medicine.disease
3. Good health
Radiation therapy
medicine.anatomical_structure
030220 oncology & carcinogenesis
Case-Control Studies
Ex vivo
Zdroj: Radiation and Environmental Biophysics
ISSN: 1432-2099
0301-634X
Popis: The aim of the study was to compare the spontaneous and ex vivo radiation-induced chromosomal damage in lymphocytes of untreated prostate cancer patients and age-matched healthy donors, and to evaluate the chromosomal damage, induced by radiotherapy, and its persistence. Blood samples from 102 prostate cancer patients were obtained before radiotherapy to investigate the excess acentric fragments and dicentric chromosomes. In addition, in a subgroup of ten patients, simple exchanges in chromosomes 2 and 4 were evaluated by fluorescent in situ hybridization (FISH), before the onset of therapy, in the middle and at the end of therapy, and 1 year later. Data were compared to blood samples from ten age-matched healthy donors. We found that spontaneous yields of acentric chromosome fragments and simple exchanges were significantly increased in lymphocytes of patients before onset of therapy, indicating chromosomal instability in these patients. Ex vivo radiation-induced aberrations were not significantly increased, indicating proficient repair of radiation-induced DNA double-strand breaks in lymphocytes of these patients. As expected, the yields of dicentric and acentric chromosomes, and the partial yields of simple exchanges, were increased after the onset of therapy. Surprisingly, yields after 1 year were comparable to those directly after radiotherapy, indicating persistence of chromosomal instability over this time. Our results indicate that prostate cancer patients are characterized by increased spontaneous chromosomal instability. This instability seems to result from defects other than a deficient repair of radiation-induced DNA double-strand breaks. Radiotherapy-induced chromosomal damage persists 1 year after treatment. peerReviewed
Databáze: OpenAIRE
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