The effect of age on the microarchitecture and profile of gene expression in femoral head and neck bone from patients with osteoarthritis
| Autor: | Mark Zhu, David S. Musson, Ian R. Reid, Nicola Dalbeth, William Caughey, Gregory D. Gamble, Ally J. Choi, Jillian Cornish, Dorit Naot, Maureen Watson, Ryan Gao, Anne Horne, Jacob T Munro, Karen E. Callon, Rocco P. Pitto |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty lcsh:Diseases of the musculoskeletal system Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Osteoarthritis Article 03 medical and health sciences Femoral head 0302 clinical medicine Bone cell Medicine Orthopedics and Sports Medicine Femur Femoral neck business.industry Bone microarchitecture medicine.disease Skeleton (computer programming) Ageing medicine.anatomical_structure Cortical bone 030101 anatomy & morphology Gene expression lcsh:RC925-935 business |
| Zdroj: | Bone Reports, Vol 13, Iss, Pp 100287-(2020) Bone Reports |
| ISSN: | 2352-1872 |
| Popis: | Ageing of the skeleton is characterised by decreased bone mineral density, reduced strength, and increased risk of fracture. Although it is known that these changes are determined by the activities of bone cells through the processes of bone modelling and remodelling, details of the molecular mechanisms that underlie age-related changes in bone are still missing. Here, we analysed age-related changes in bone microarchitecture along with global gene expression in samples obtained from patients with osteoarthritis (OA). We hypothesised that changes would be evident in both microarchitecture and gene expression and aimed to identify novel molecular mechanisms that underlie ageing processes in bone. Samples of femoral head and neck were obtained from patients undergoing hip arthroplasty for OA, who were either ≤60 years or ≥70 years of age. Bone microarchitecture was analysed in cores of trabecular bone from the femoral head (17 from the younger group and 18 from the older), and cortical bone from the femoral neck (25 younger/22 older), using a Skyscan 1172 microCT scanner (Bruker). Gene expression was compared between the two age groups in 20 trabecular samples from each group, and 10 cortical samples from each group, using Clariom S Human microarrays (ThermoFisher Scientific). We found no significant changes between the two age groups in indices of trabecular or cortical bone microarchitecture. Gene expression analysis identified seven genes that had higher expression in the older group, including the transcription factor EGR1 and the glucose transporter SLC2A3 (GLUT3), and 21 differentially expressed genes in cortical bone samples (P2). However, none of the comparisons of gene expression had false discovery rate-adjusted P Highlights • Trabecular and cortical bone from people with OA 70 years old were studied. • Bone microarchitecture and gene expression were similar in the two age groups. • Pathological processes related to OA may protect against age-related bone loss. |
| Databáze: | OpenAIRE |
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