Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
| Autor: | W. Samuel Fagg, Adam Hage, Mark T. Bedford, Shelton S. Bradrick, Vineet D. Menachery, Minato Hirano, K. Reddisiva Prasanth, Pei Yong Shi, Chao Shan, Eileen T. McAnarney, Ricardo Rajsbaum, Mariano A. Garcia-Blanco, Xuping Xie, Alexander Bukreyev, Colette Pietzsch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses 030106 microbiology Biology Virus Replication Virus Article Cell Line Dengue fever Betacoronavirus Gene Knockout Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Virology Replication (statistics) medicine Humans RNA Viruses Topoisomerase III Host factor 030304 developmental biology Pharmacology 0303 health sciences SARS-CoV-2 Topoisomerase Yellow fever RNA Zika Virus Dengue Virus Ebolavirus medicine.disease 3. Good health 030104 developmental biology DNA Topoisomerases Type I chemistry Cell culture Influenza A virus biology.protein Yellow fever virus 030217 neurology & neurosurgery DNA |
| Zdroj: | Antiviral Research bioRxiv article-version (status) pre article-version (number) 1 |
| DOI: | 10.1101/2020.03.24.005900 |
| Popis: | Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target. Highlights • Topoisomerase III-ß (TOP3B), which is the only human topoisomerase that can act on both DNA and RNA, is a host factor for all single stranded positive strand RNA viruses. • TOP3B is not a host factor for Ebola and influenza viruses, which are negative strand RNA viruses. • TOP3B acts directly on the viral genome of DENV2. • TOP3B could be a target for antiviral development. |
| Databáze: | OpenAIRE |
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