Diminished serum Gc (vitamin D-binding protein) levels and increased Gc:G-actin complexes in a hamster model of fulminant hepatic necrosis
| Autor: | David L. Emerson, David J. Jollow, Pascal J. Goldschmidt-Clermont, William O. Young, Robert M. Galbraith, William M. Lee |
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| Rok vydání: | 1987 |
| Předmět: |
Male
Vitamin Immunodiffusion medicine.medical_specialty Vitamin D-binding protein Hamster Immunoelectrophoresis Biology Necrosis chemistry.chemical_compound Liver disease Cricetinae Internal medicine medicine Animals Homeostasis Aspartate Aminotransferases Acetaminophen Liver injury Diminution Mesocricetus Hepatology medicine.diagnostic_test Liver Diseases Vitamin D-Binding Protein medicine.disease Actins Endocrinology Liver chemistry Chemical and Drug Induced Liver Injury medicine.drug |
| Zdroj: | Hepatology. 7:825-830 |
| ISSN: | 1527-3350 0270-9139 |
| Popis: | Evidence for increased plasma levels of complexes containing Gc (vitamin D-binding protein) and cellular actin has been previously reported during fulminant hepatic necrosis in man. In order to study this process in more detail, we produced liver injury in hamsters using increasing doses of acetaminophen, with serial collection of sera for up to 168 hr after acetaminophen injection. Hamster Gc was purified using a three-step procedure and was shown to resemble closely human Gc. Polyclonal antihamster Gc was prepared and used in rocket immunoelectrophoresis and radial immunodif-fusion studies for quantitation of total serum Gc and the percentage of Gc complexed with actin. Serum Gc levels were depressed in animals having liver damage, and the extent of depression 42 hr after acetaminophen correlated with the extent of elevation of AST. The proportion of the total Gc that was present in the complexed form increased in relation to the severity of the liver disease. In serial studies, diminution in Gc level preceded the rise in AST and increase in the percent complexed. These changes closely resemble observations in man and suggest that the hamster-acetaminophen hep-atotoxicity model may be of value in further study of interactions of Gc with intracellular actin components and its role in actin homeostasis in conditions of massive tissue necrosis. |
| Databáze: | OpenAIRE |
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