The nature of the human blood group P1 determinant
| Autor: | Walter Thomas James Morgan, Helene T. Cory, A.S.R. Donald, Winifred M. Watkins, A.D. Yates |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Biophysics
Oligosaccharides Biochemistry Hydrolysis Sodium borohydride chemistry.chemical_compound Echinococcosis Glucosamine Agglutination Tests Animals Humans Molecular Biology Glycoproteins chemistry.chemical_classification Sheep Chromatography Galactosidases Galactose Cell Biology Oligosaccharide Paper chromatography chemistry Blood Group Antigens Chromatography Gel Rabbits Glycoprotein |
| Zdroj: | Biochemical and Biophysical Research Communications. 61:1289-1296 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(74)80424-9 |
| Popis: | Summary A glycoprotein with blood group P 1 specificity isolated from sheep hydatid cyst fluid was subjected to partial acid hydrolysis. The hydrolysis products were separated by preparative paper chromatography. One oligosaccharide with strong P 1 serological activity was characterised as a trisaccharide composed of galactose and glucosamine in the molar ratio of 2:1. Reduction with sodium borohydride gave galactose and glucosaminitol. Methylation analysis and degradation with specific α - and β - galactosidases showed the structure of the P 1 determinant to be D-galactosyl-α(l→4)-D-galactosyl-β(l→4)-N-acetyl-D-glucosamine. |
| Databáze: | OpenAIRE |
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