Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Autor: Aleksandra Bielawska-Pohl, Piotr Jamroz, Jerzy Dora, Claudine Kieda, Anna Dzimitrowicz, Aleksandra Klimczak, Giovanni Busco, Agnieszka Krawczenko, Pawel Pohl, Aleksandra Baszczynska, Dominik Terefinko, Catherine Grillon
Přispěvatelé: Wroclaw University of Science and Technology, Hirszfeld Institute of Immunology and Experimental Therapy, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), GRILLON, Catherine, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Military Institute of Medicine, Warsaw
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Work (thermodynamics)
Materials science
General Chemical Engineering
Proliferation
Wound healing
Atmospheric-pressure plasma
Human skin
[SDV.CAN]Life Sciences [q-bio]/Cancer
Dielectric barrier discharge
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
01 natural sciences
03 medical and health sciences
Search engine
[SDV.CAN] Life Sciences [q-bio]/Cancer
0103 physical sciences
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Response surface methodology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Migration
030304 developmental biology
010302 applied physics
0303 health sciences
General Chemistry
[SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology
Condensed Matter Physics
Surfaces
Coatings and Films
HaCaT
Cold atmospheric pressure plasma
Angiogenesis
[SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Biomedical engineering
Zdroj: Plasma Chemistry and Plasma Processing
Plasma Chemistry and Plasma Processing, 2019, ⟨10.1007/s11090-019-10039-0⟩
Plasma Chemistry and Plasma Processing, Springer Verlag, 2019, ⟨10.1007/s11090-019-10039-0⟩
ISSN: 0272-4324
1572-8986
DOI: 10.1007/s11090-019-10039-0⟩
Popis: Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature Trot(OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the Trot(OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds.
Databáze: OpenAIRE
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