Identification of a surface protective antigen, MAP of Streptococcus equi subspecies zooepidemicus
| Autor: | Huihuang Liang, Lili Yan, Mingyong Deng, Xue-jiao Gao, Bin Tang, Zigong Wei, Pan Zhai, Huahao Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
040301 veterinary sciences
medicine.drug_class Protein subunit Antibiotics Biology Microbiology law.invention 0403 veterinary science Mice 03 medical and health sciences Bacterial Proteins Antigen Western blot law Streptococcal Infections medicine Animals Streptococcus equi 030304 developmental biology Antigens Bacterial Mice Inbred BALB C 0303 health sciences General Veterinary medicine.diagnostic_test Lethal dose 04 agricultural and veterinary sciences biology.organism_classification Antigens Surface Inactivated vaccine Recombinant DNA Female Bacteria |
| Zdroj: | Research in Veterinary Science. 124:387-392 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2019.04.021 |
| Popis: | Streptococcus equi subspecies zooepidemicus (SEZ) is a zoonotic pathogen with adhesive and invasive properties. Due to the shortcomings of antibiotics and traditional inactivated vaccine, identifying protective antigens against SEZ would be helpful to the development of novel vaccines. MAP has been identified as a membrane anchored protein with a typical LPXTG-like cell wall-anchored motif. In present study, the objective was to evaluate the effects of MAP as a subunit vaccine with mouse model. The Western blot analysis shown that the purified recombinant MAP presented good immunoreactive to convalescent porcine sera against SEZ. The protein could elicit a remarkable humoral antibody response and protect 80% of mice against lethal dose challenge of SEZ in mouse model. Moreover, the hyperimmune sera against MAP could efficiently kill the bacteria in whole blood killing assay and conferred significant protection against SEZ in passive immunization experiments. This study suggests with good reasons that MAP could be a novel and effective vaccine candidate for SEZ. |
| Databáze: | OpenAIRE |
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