Performance studies of an enzyme-linked immunosorbent assay for detecting Staphylococcus aureus antibody in bovine milk
| Autor: | D. S. Adams, P. M. Sears, R. P. Dinsmore, R. J. Eberhart, J. S. McDonald, T. Matsushita, G. M. Jones |
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| Rok vydání: | 1990 |
| Předmět: |
0301 basic medicine
Bovine milk Staphylococcus aureus 040301 veterinary sciences 030106 microbiology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Microbiology 0403 veterinary science 03 medical and health sciences Predictive Value of Tests medicine Animals Mastitis Bovine chemistry.chemical_classification General Veterinary biology business.industry Reproducibility of Results 04 agricultural and veterinary sciences Staphylococcal Infections medicine.disease biology.organism_classification Antibodies Bacterial Mastitis Enzyme Staphylococcus aureus Antibody Milk chemistry Immunology biology.protein Cattle Female Antibody business Bacteria Inflammatory disorder |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 2(3) |
| ISSN: | 1040-6387 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) for detecting Staphylococcus aureus antibody in bovine milk samples was examined for repeatability. A set of 51 bovine milk samples from 4 universities with confirmed culture results was assembled, and a panel of 30 milk samples was randomly selected. When the selected panel was tested at the collection laboratory, there was 97% agreement between the ELISA and the culture test. The panel was tested with the ELISA by the 4 university laboratories. Results were scored by both visual and optical density reader methods. When compared to reference ELISA results, the university laboratory ELISA results showed an agreement of 99.8% for negative samples, 98% for positive samples, and 99% for all samples. Additional studies on 19 milk samples that cultured positive for bacteria other than S. aureus showed 100% specificity. Overall comparison of ELISA and culture results showed high agreement between the 2 techniques. Disagreement appeared to result from explainable differences in antibody and bacterial levels and not from errors in either of the 2 techniques. Mastitis is an inflammatory disorder of the mam- mary gland that is the most costly disease of dairy cows. Most cases of contagious mastitis are caused by the bacterium Staphylococcus aureus. 3 Recently, an en |
| Databáze: | OpenAIRE |
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