Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant
| Autor: | Maura C. Cannon, Alexandra N. Frye, Yuning Chen, Marcia J. Kieliszewski, Andrew J. Mort, Michael A. Held, Dening Ye, Prasenjit Saha, Tui Ray |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mutant
Plant Science LRX Article Cell wall proteomics Arabidopsis lcsh:Botany PRP4 Gene Extensin Ecology Evolution Behavior and Systematics Gene knockout chemistry.chemical_classification Ecology biology Wild type biology.organism_classification Cell biology lcsh:QK1-989 Arabidopsis root Biochemistry chemistry hydroxyproline-rich glycoprotein biology.protein cell wall Extensin 3 Glycoprotein |
| Zdroj: | Plants, Vol 4, Iss 1, Pp 85-111 (2015) Plants Volume 4 Issue 1 Pages 85-111 |
| ISSN: | 2223-7747 |
| Popis: | Extensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, although about 20% of the knockout plants have an apparently normal phenotype (ANP). In this study the root cell wall HRGP components of wild-type, ANP and the ext3 mutant seedlings were characterized by peptide fractionation of trypsin digested anhydrous hydrogen fluoride deglycosylated wall residues and by sequencing using LC-MS/MS. Several HRGPs, including EXT3, were identified in the wild-type root walls but not in walls of the ANP and lethal mutant. Indeed the ANP walls and walls of mutants displaying the lethal phenotype possessed HRGPs, but the profiles suggest that changes in the amount and perhaps type may account for the corresponding phenotypes. |
| Databáze: | OpenAIRE |
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