Examination of a Chinese-made cryptococcal glucuronoxylomannan antigen test in serum and bronchoalveolar lavage fluid for diagnosing pulmonary cryptococcosis in HIV-negative patients
| Autor: | Pei-Ying Zeng, Ming-Die Wang, Zhaoming Chen, Zhengtu Li, Shaoqiang Li, Yangqing Zhan, Feng Ye, Jing Cheng, Ye Lin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty China Antigens Fungal 030106 microbiology Human immunodeficiency virus (HIV) Chest ct HIV Infections medicine.disease_cause Positive correlation Gastroenterology 03 medical and health sciences 0302 clinical medicine Antigen Polysaccharides Internal medicine medicine Immunology and Allergy Humans 030212 general & internal medicine Pulmonary cryptococcosis General Immunology and Microbiology medicine.diagnostic_test business.industry General Medicine Cryptococcosis Antigen test Serum samples Infectious Diseases Bronchoalveolar lavage business Bronchoalveolar Lavage Fluid |
| Zdroj: | Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi. 55(2) |
| ISSN: | 1995-9133 |
| Popis: | We presented the performance of a Chinese-made cryptococcal glucuronoxylomannan (GXM) antigen test using serum and bronchoalveolar lavage fluid (BALF) samples in the HIV-negative Chinese population.Between February 2017 and January 2019, HIV-negative patients with pulmonary cryptococcosis were recruited and followed-up every three months, including completion of a chest CT examination and collection of serum and BALF samples.Here, thirty-seven confirmed and ten clinically diagnosed patients were recruited. Furthermore, samples from 174 noncryptococcosis patients that may cause false positives were also collected. The sensitivity of a lateral flow assay (LFA) for detecting cryptococcal GXM antigen in serum and BALF samples from confirmed cases was 97% and 95%, respectively, and the specificity was 98.2% and 93%, respectively, and the differences in these values between the BALF and serum samples were not significant. The serum cryptococcal GXM antigen value showed a positive correlation (r: 0.581, p 0.001) with pulmonary lesion size, while the BALF value showed no correlation (r: 0.253, p: 0.13). The positivity rate of BALF was higher than that of serum when the diameter of the pulmonary lesion was small (diameter less than 20 mm). Moreover, the serum cryptococcal GXM antigen levels showed an overall decreasing trend with the decrease in pulmonary lesion size after antifungal therapy in patient follow-up.The Chinese-made cryptococcal GXM antigen test has better sensitivity and specificity for diagnosing pulmonary cryptococcosis in the HIV-negative Chinese population, and it could be used to diagnose and to monitor this disease. |
| Databáze: | OpenAIRE |
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