Phenotypic characterization of taste cells of the mouse small intestine
| Autor: | Nicole J. Cooper, L. Ashley Blackshaw, Kate Sutherland, Michael Horowitz, Richard L. Young |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Serotonin endocrine system medicine.medical_specialty Taste Physiology Ratón Enteroendocrine Cells Gene Expression Enteroendocrine cell Nitric Oxide Synthase Type I Biology Mice chemistry.chemical_compound stomatognathic system Glucagon-Like Peptide 1 Physiology (medical) Internal medicine Intestine Small medicine Animals Transducin Intestinal Mucosa Neurotransmitter Hepatology Gastroenterology Immunohistochemistry Small intestine Cell biology Mice Inbred C57BL Phenotype medicine.anatomical_structure Endocrinology chemistry Gastrointestinal hormone Specialized Epithelial Cell Plant Lectins |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 292:G1420-G1428 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00504.2006 |
| Popis: | Nutrient-evoked gastrointestinal reflexes are likely initiated by specialized epithelial cells located in the small intestine that detect luminal stimuli and release mediators that activate vagal endings. The G-protein alpha-gustducin, a key signal molecule in lingual taste detection, has been identified in mouse small intestine, where it may also subserve nutrient detection; however, the phenotype of alpha-gustducin cells is unknown. Immunohistochemistry was performed throughout the mouse small intestine for alpha-gustducin, enteroendocrine cell markers 5-HT and glucagon-like peptide-1 (GLP-1), and brush cell markers neuronal nitric oxide synthase and Ulex europaeus agglutinin-1 (UEA-1) lectin binding, singly, and in combination. alpha-Gustducin was expressed in solitary epithelial cells of the mid to upper villus, which were distributed in a regional manner with most occurring within the midjejunum. Here, 27% of alpha-gustducin cells colabeled for 5-HT and 15% for GLP-1; 57% of alpha-gustducin cells colabeled UEA-1, with no triple labeling. alpha-Gustducin cells that colabeled for 5-HT or GLP-1 were of distinct morphology and exhibited a different alpha-gustducin immunolabeling pattern to those colabeled with UEA-1. Neuronal nitric oxide synthase was absent from intestinal epithelium despite strong labeling in the myenteric plexus. We conclude that subsets of enteroendocrine cells in the midjejunum and brush cells (more generally distributed) are equipped to utilize alpha-gustducin signaling in mice. Intestinal taste modalities may be signaled by these enteroendocrine cells via the release of 5-HT, GLP-1, or coexpressed mediators or by brush cells via a nonnitrergic mediator in distinct regions of the intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|