Promoter analysis of the catalase-peroxidase gene (cpeA) fromRhodobacter capsulatus
| Autor: | Monier H. Tadros, Gerhart Drews, Hubert Forkl |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Recombinant Fusion Proteins Molecular Sequence Data lac operon Microbiology Rhodobacter capsulatus Primer extension Bacterial Proteins Start codon Transcription (biology) Gene expression Escherichia coli Genetics Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Sequence Deletion Regulation of gene expression Rhodobacter Base Sequence biology Chromosome Mapping Gene Expression Regulation Bacterial Hydrogen Peroxide biology.organism_classification Molecular biology Lac Operon Peroxidases Genes Bacterial |
| Zdroj: | FEMS Microbiology Letters. 137:169-174 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1996.tb08101.x |
| Popis: | The expression of the Rhodobacter capsulatus catalase-peroxidase gene (cpeA) was studied by in-frame fusions of the upstream region of the cpeA gene to a promoter-less lacZ gene. The transcription of the cpeA gene is about 20-50-fold higher under aerobic-dark than under anaerobic-light conditions. The promoter was localized within a 69-bp upstream DNA region. The transcription start site, determined by primer extension, is 28 bases upstream from the initiation codon, confirming the postulated promoter localized by deletion analysis. Deletion of the part of the upstream region specifically responsible for oxygen regulation resulted in constitutive expression of the cpeA gene. |
| Databáze: | OpenAIRE |
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