Use of the metal-ion indicator, Arsenazo III, in the measurement of calcium binding
| Autor: | W.B. Gratzer, G.H. Beaven |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Inorganic chemistry
Biophysics Analytical chemistry chemistry.chemical_element Calcium Biochemistry Arsenicals Ion Metal Molecular Biology Edetic Acid Binding Sites Ternary numeral system Chemistry Arsenazo III Cell Biology Kinetics Spectrophotometry Free calcium visual_art visual_art.visual_art_medium Ph range Indicators and Reagents Azo Compounds Macromolecule |
| Zdroj: | Analytical Biochemistry. 81:118-129 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(77)90604-2 |
| Popis: | The use of the metal-ion indicator, Arsenazo III, for the spectrophotemetric measurement of total and free calcium concentrations has been explored. The indicator can be used satisfactorily in the physiological pH range. A procedure is described for the direct measurement of calcium-binding equilibria in a ternary system containing the indicator, calcium ions, and a calcium-binding macromolecule. This appears to offer some advantages over conventional methods in situations in which the total calcium-binding capacity is low. Results on two proteins are reported, and the limitations of the method are discussed. |
| Databáze: | OpenAIRE |
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