Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase - a kinetic and DFT study
| Autor: | Lena J. Daumann, Huub J. M. Op den Camp, Henning Lumpe, Arjan Pol |
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| Rok vydání: | 2018 |
| Předmět: |
Lanthanide
Lanthanide contraction biology Methanol dehydrogenase 010405 organic chemistry chemistry.chemical_element Bioinorganic chemistry 010402 general chemistry 01 natural sciences Medicinal chemistry Lutetium 0104 chemical sciences Inorganic Chemistry chemistry Ecological Microbiology Lanthanum biology.protein Lewis acids and bases Alcohol dehydrogenase |
| Zdroj: | Dalton Transactions, 47, 10463-10472 Dalton Transactions, 47, 31, pp. 10463-10472 Dalton Transactions |
| ISSN: | 1477-9226 |
| Popis: | Interest in the bioinorganic chemistry of lanthanides is growing rapidly as more and more lanthanide-dependent bacteria are being discovered. Especially the earlier lanthanides have been shown to be preferentially utilized by bacteria that need these Lewis acids as cofactors in their alcohol dehydrogenase enzymes. Here, we investigate the impact of the lanthanide ions lanthanum(iii) to lutetium(iii) (excluding Pm) on the catalytic parameters (vmax, KM, kcat/KM) of a methanol dehydrogenase (MDH) isolated from Methylacidiphilum fumariolicum SolV. Kinetic experiments and DFT calculations were used to discuss why only the earlier lanthanides (La-Gd) promote high MDH activity. Impact of Lewis acidity, coordination number preferences, stability constants and other properties that are a direct result of the lanthanide contraction are discussed in light of the two proposed mechanisms for MDH. |
| Databáze: | OpenAIRE |
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