Caspase-3, a shrimp phosphorylated hemocytic protein is necessary to control YHV infection

Autor: Sittiruk Roytrakul, Phattara-orn Havanapan, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Chartchai Krittanai, Atchara Paemanee, Nuanwan Phungthanom
Rok vydání: 2021
Předmět:
Zdroj: Fish & Shellfish Immunology. 114:36-48
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2021.04.007
Popis: By using immunohistochemistry detection, yellow head virus (YHV) was found to replicate in granule-containing hemocytes including semi-granular hemocytes (SGC) and granular hemocytes (GC) during the early phase (24 h post injection) of YHV-infected shrimp. Higher signal of YHV infection was found in GC more than in SGC. Comparative phosphoproteomic profiles between YHV-infected and non-infected GC reveal a number of phosphoproteins with different expression levels. The phosphoprotein spot with later on identified as caspase-3 in YHV-infected GC is most interesting. Blocking caspase-3 function using a specific inhibitor (Ac-DEVD-CMK) demonstrated high replication of YHV and consequently, high shrimp mortality. The immunohistochemistry results confirmed the high viral load in shrimp that caspase-3 activity was blocked. Caspase-3 is regulated through a variety of posttranslational modifications, including phosphorylation. Analysis of phosphorylation sites of shrimp caspase-3 revealed phosphorylation sites at serine residue. Taken together, caspase-3 is a hemocytic protein isolated from shrimp granular hemocytes with a role in anti-YHV response and regulated through the phosphorylation process.
Databáze: OpenAIRE
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